【作者】 薛汉荣； 余建玮； 程光宇； 付向春；

【Author】 YU Jian-wei~1,XUE Han-rong~1,CHENG Guang-yu~1,FU Xiang-chun~1,(1 Institute of Respiration Disease,Jiangxi College of Traditional Chinese Medicine Nanchang 330006,)

【机构】 江西中医学院呼吸病研究所；

【摘要】 目的:探索建立符合中医寒哮临床发病特点的动物模型。方法:50只清洁级健康雄性SD大鼠随机分为正常对照组(A组)、西医对照组(B组)、中医模型1组(C组)、中医模型2组(D组)和中医模型3组(E组),每组10只。检测各组大鼠肺泡灌洗液(BALF)中细胞变化、BALF中IL-4、IFN-γ水平变化以及大鼠肺组织病理学的改变。结果:(1)B、C、D、E组BALF中细胞总数和嗜酸性粒细胞(EOS)百分比均较正常组A组升高(P<0.01),且C组大鼠BALF中的细胞总数和EOS所占比例明显高于B组(P<0.01)。(2)B、C、D、E组大鼠BALF中IFN-γ的含量与正常组A组比较均降低(P<0.01),且C组与B组组间比较存在明显的差异(P<0.05)。B、C、D、E组大鼠BALF中IL-4的含量与A组比较均升高(P<0.01);C组、D组、E组与B组IL-4的含量差异均具有统计学意义(P<0.05),且以C组的差异最为明显(P<0.01),同时D组、E组与C组组间比较具有显著性差异(P<0.05)。(3)中医模型组大鼠都表现哮喘炎症反应的病理改变。结论:大鼠BALF中的EOS百分比、IL-4和INF-γ浓度及病理的改变可能成为中医寒哮动物模型的主要评价指标。更多还原

【Abstract】 Objective:To explore the establishment of animal model consistent with the traditional Chinese medicine clinical morbility feature of the cold asthma.Method:50 Clean healthy male SD rats were randomly divided into 5 groups:the normal control group(Group A),Western medicine control group (Group B),Traditional Chinese Medicine(TCM) mode 1 group(Group C),TCM mode 2 group(Group D) and TCM mode 3 group(Group E),10 in each group.Test the changes in cells of rats in BALF,the changes in levels of IL-4,IFN-γin BALF and also the changes in histopathology of rat lung.The results: (l)the total number of cells and percentage of EOS in BALF of Group B,C,D,E were higher than that of normal control group(P<0.01),and the total number of cells and percentage of EOS in BALF of Group C rats was higher significantly than that of Group B(P<0.01).(2) The content of IFN-γin BALF of Group B, C,D,E rats were lower than that of Group A(P<0.01),and Group comparison of Group C and Group B is existence significant difference(P<0.05).The contents of IL-4 in BALF of Group B,C,D,E rats were higher than that of Group A(P<0.01);the content of IL-4 of TCM Model Group C,Group D,Group E comparison with Group B were significant differences statistically(P<0.05),and the differences of Group C were the most obvious in of all(P<0.01),Chinese medicine model Group D,Group E comparison with Group C were significantly different(P<0.05).(3) rats of model group have shown the pathological changes of asthma inflammatory response.Conclusion:Changes of eosinophil percentage, concentration of IL-4 and INF-γand pathological in BALF of rat may be the main evidence of evaluation to TCM animal model of the cold asthma.更多还原