【作者】 胡周敏； 卢德赵； 沃兴德； 楼丽华； 赵虹；

【Author】 HU Zhou-min~1,LU De-zhao~1,WO Xing-de~1,LOU Li-hua~2,ZHAO Hong~2 (1 College of Life Science,Zhejiang Chinese Medical university,Hangzhou 310053,China;2 Research center of Mammary gland,Zhejiang hospitalof Traditional Chinese medicine,Hangzhou,310006 China)

【机构】 浙江中医药大学生命科学学院； 浙江省中医院乳腺病中心；

【摘要】 目的:建立具有高分辨率和稳定性的乳腺纤维瘤组织蛋白质组的双向电泳图谱,并对其进行差异蛋白质组分析。方法:取乳腺纤维瘤病患者病变部位及正常乳腺组织,匀浆提取乳腺组织总蛋白,分别用Cy3或Cy5标记,每一对cy3和Cy5标记样品都与一个Cy2标记的内标等量混合,上样于同一胶中进行电泳分离,经不同光激发下扫描得到不同样品的蛋白质组图谱。所获得的图谱经DeCyder软件分析。结果:在乳腺纤维瘤病的病变组织中,有37个蛋白质表达水平显著增加,另外8个蛋白质表达水平显著下降。结论:分析所得的45个差异蛋白质可能与乳腺纤维瘤疾病的发生与发展有关。更多还原

【Abstract】 Objective To establish two—dimensional differential gel electrophoretogram of breast adenof ibroma with high resolution capacity and stability and process the analysis of differential proteome,Methods Total protein of Galactophore tissue was extracted from the breast adenofibroma tissue and normal breast tissue by the method of homogenization.The extracts were labeled with Cy3 or Cy5 randomly.Each pair of Cy3-labeled sample and Cy5-labeled sample was mixed on the same 2-D gel along with a Cy2-labeled mixture of all samples as an internal standard.The gels were imaged using different emission filter respectively and spot—features were analyzed by DeCyder software in each paired normal/disease comparison.Results 37 unique proteins increased remarkably more than 2 folders up while 8 proteins decreased more than 2 folders down in breast adenofibroma tissue than in normal breast tissue.Conclusions these 45 differential proteins from this analysis might be involved in the process ofbreast adenofibroma.更多还原