【作者】 祁贤； 崔仑标； 李亮； 汤奋扬； 郭喜玲； 焦永军； 单军； 吴斌； 祖荣强； 邓斐； 付建光； 秦圆方； 王慎骄； 霍翔； 许可； 朱凤才； 史智扬； 羊海涛； 汪华；

【Author】 QI Xian,CUI Lun-biao,LI Liang,TANG Fen-yang,GUO Xi-lin, JIAO Yong-jun,SHAN Jun,WU Bin,ZU Rong-qiang,DENG Fei,FU Jian-guang,QIN Yuan-fang, WANG Shen-jiao,HUO Xiang,XU Ke,ZHU Feng-cai,SHI Zhi-yang,YANG Hai-tao,WANG Hua Jiangsu Center for Disease Control and Prevention,Nanjing 210009,China

【机构】 江苏省疾病预防控制中心；

【摘要】 目的探讨甲型H1N1流感病毒的遗传特征。方法 2009年6月12日,江苏确诊首例甲型H1N1病例。通过细胞和鸡胚分离系统,分离到一株具有较高血凝活性的病毒,命名为A/Jiangsu/1/2009。为了跟踪病毒的变异情况,随继开展了病毒的全基因组测序工作,在此基础上对其血凝素基因(haemagglutinin,HA)的遗传特性进行了详细研究。结果分离株HA蛋白不具有多碱基HA裂解位点,具有低致病性流感病毒特点。与参考株A/California/04/2009相比,分离株A/Jiangsu/1/2009 HA蛋白有4个氨基酸发生了突变,但都不在已知的抗原位点上。分离株有5个潜在糖基化位点,这与近年来古典猪H1N1和北美三源重配猪H1病毒完全一致,保留了古典猪H1的特点。结论与禽流感H1病毒相比,分离株HA蛋白受体结合位点上的E190D和G225D发生突变,这可能成为新甲型H1N1在人际间传播的一个重要分子基础。此外,其它受体结合位点上相关氨基酸同时具有人和猪流感病毒的特点。本研究首次对早期流行的甲型H1N1流感病毒的HA蛋白的分子遗传特征进行了详细研究,对进一步监测病原变异具有重要指导意义。更多还原

【Abstract】 Obeltive To explore the genetic characterization of Influenza A H1N1 Methods On June 12,2009,the first case of novel influenza A H1N1(2009) virus infection was confirmed in Jiangsu Province. A virus with hemagglutination activity was isolated from the clinical samples derived from the patient using both MDCK cells and embryonic eggs,which was designated as A/Jiangsu/1/2009(H1N1).In order to monitor evolution of the virus,we carried out whole-genome sequencing,and analyzed the genetic characteristic of hemagglutinin(HA) of the isolate in detail.Results The results showed that HA did not contain the multibasic amino acid motif at cleavage sites,which is a characteristic of low pathogenic influenza viruses.Compared to reference virus A/California/04/2009,the HA protein of the isolate had four amino acid mutation,which were not located on the known antigenic sites.Consistent with the triple-reassortant swine H1 viruses from Northern America in recent years,the isolate contained five potential glycosylation sites,which also was the feature of classical swine H1N1 viruses.Compared with avian H1 viruses, two amino acid(E190D and G225D) were changed on receptor binding sites,which might be an important molecular mechanism of human-to-human transmission for the novel viruses.Conclusions In this study,for the first time,we studied the molecular characteristics of the HA protein of the novel influenza A H1N1(2009) viruses in detail,which have an important guiding significance for further monitoring of virus mutation.更多还原