【作者】 黄云梅； 陈文列； 胡海霞； 黄祖芳； 黄美雅； 郑良朴； 林如辉； 李钻芳； 杜建；

【Author】 HUANG Yunmei,~*CHEN Wenlie,HU Haixia,HUANG Zufang,HUANG Meiya,ZHENG Liangpu,LIN Ruhui,DU Jian 1.Key Laboratory of Fujian Academy of Integrative Medicine,Fuzhou 350108,China 2.Fujian Key Laboratory of Integrative Medicine on Geriatrics(Fujian University of TCM),Fuzhou350108,China 3.The Key Laboratory of Basics of Integrative Medicine of Fujian Higher Education(Fujian University of Traditional Chinese Medicine),Fuzhou 350108,China

【机构】 福建中西医结合研究院； 福建省中西医结合老年性疾病重点实验室(福建中医学院)； 福建省高校中西医结合基础重点实验室(福建中医学院)； 国家中医药管理局三级科研实验室-中药药理(细胞结构与功能)实验室； 医学光电科学与技术教育部重点实验室(福建师范大学)；

【摘要】 目的:探讨中药复方解毒消症饮对人胃癌细胞凋亡相关基因的影响。方法:将培养的人胃腺癌细胞株BGC-823分成低、中、高剂量含药血清组及空白血清对照组,分别加入相应剂量的解毒消症饮大鼠含药血清和空白对照血清,培养24h、48h和72h;免疫荧光染色法检测细胞凋亡相关基因Bax、Bcl-2、Fas及Fas-L的表达,应用激光共聚焦显微镜观察并做半定量分析。结果:胃癌细胞经解毒消症饮含药血清干预后,Bax、Fas表达明显增加(P<0.05),且随药物浓度的增加该趋势更加明显,Fas-L则随药物浓度的增加表达明显减弱(P<0.05);各含药血清组与空白血清对照组相比,Bcl-2的表达量无明显变化。结论:解毒消症饮可能通过调控胃癌细胞Bax、Fas及Fas-L等基因,从而促进细胞凋亡,并降低细胞免疫逃逸的功能。更多还原

【Abstract】 Objective:To study the effect of compound Chinese medicine(Jiedu-Xiaozheng-Yin) on apoptosis related genes of human gastric carcinoma cells.Methods:Cultured human gastric carcinoma cells BGC-823 was divided into four groups:low-dose,medium-dose,high-dose of drug serum group and control group,each group treated with corresponding rat serum containing Jiedu-Xiaozheng-Yin or control serum.The cells were cultured for 24 hours,48 hours or 72 hours, then Immunofluorescent staining was performed to detect the expression of Bax,Bcl-2,Fas and Fas-L in the cells that results were observed and semiquantitativly analysed by laser scanning confocal microscopy.Results:Compared with the control group,the expression of Bax and Fas was significantly increased(P<0.05),while the expression of Fas-L was significantly decreased (P<0.05) after treated with drug serum,and both in a dose dependent manner.The drug serum made no difference on the expression of Bcl-2.Conclusion:Compound Chinese medicine (Jiedu-Xiaozheng-Yin) can regulate the levels of Bax,Fas and Fas-L in gastric carcinoma cells,then promote cell apoptosis and weaken immune escape.更多还原