【作者】 李冰； 张继瑜； 周绪正； 李金善； 李剑勇； 杨亚军； 牛建荣； 魏小娟；

【Author】 LI Bing,ZHANG Ji-yu,ZHOU Xu-zheng,LI Jin-shan,LI Jian-yong, YANG Ya-jun,NIU Jian-rong,WEI Xiao-juan (Lanzhou Institute of Animal Science and Veterinary Pharmaceutics,Key Laboratory of New Animal Drug Project,Chinese Academy of Agricultural Sciences,Lanzhou,730050 )

【机构】 中国农业科学院兰州畜牧与兽药研究所； 中国农业科学院新兽药工程重点开放实验室；

【摘要】 目的建立氢溴酸槟榔碱片剂的HPLC-MS/MS含量测定方法.方法采用ZorbaxSBC18柱(2.1×30mm,3.5um)分析柱,以甲醇-甲酸铵水溶液(pH4.5)(15:85)为流动相,流速为0.4 mL·min-1;采用ESI+MRM进行离子方式监测.用于定量分析的母/子离子对为m/z 156.2—53.0(槟榔碱).结果氢溴酸槟榔碱片剂检测方法的线性范围为5-120ng·mL-1,最低检测限为5ng·mL-1.平均加样回收率为99.36%,RSD为1.06%,日内、日间精密度RSD均小于3%.结论本方法灵敏、准确,可用于氢溴酸槟榔碱片剂的含量测定及质量标准的制定.更多还原

【Abstract】 Objective To develop an HPLC- MS/MS method for determination of arecoline hydrobromide tablet.Methods The analysis was performed on a Zorbax SB C18(2.1×30mm> 3.5um)analytical column using MeOH- ammonium formate (pH4.5)(15:85)as the mobile phase at a flow rate of 0.4 mL·min-1.The analysis were detected by the electronic-spray ionization(ESI)-multiple reaction monitoring(MRM)mode of the transitions of m/z 156.2→53.0 for arecoline.Results The linear range of arecoline was5-120ng·mL-1 and limit of detection was 5 ng·mL-1.The average recovery was 99.36%with RSD1.06%.The RSD%of intra-day and inter-day were less than 3%.Conclusion The method was simple,rapid,and accurate and suitable for the detection of arecoline hydrobromide tablet.更多还原