【作者】 马霞； 郭振环； 沈志强； 胡元亮；

【Author】 Guo Zhen-huan,Ma Xia,Shen Zhi-qiang~* (Shandong Province Binzhou Animal Science and Veterinary Medicine Academy,Binzhou,Shandong 256600)

【机构】 南京农业大学中兽医学研究室； 山东省滨州畜牧兽医研究院；

【摘要】 比较蜂胶佐剂和油乳剂及铝胶对猪细小病毒灭活疫苗的佐剂活性.对豚鼠进行免疫应答试验,检测血清抗体水平,脾脏淋巴细胞增殖转化和细胞上清液IL-2及IL-4含量.结果显示:蜂胶、油乳剂和铝胶三种佐剂均能提高豚鼠对猪细小病毒灭活疫苗的免疫应答能力,油乳剂提高血清HI抗体效果较好,其次为蜂胶佐剂,再次为铝胶佐剂.蜂胶佐剂促进T淋巴细胞增殖和提高IL-2、IL-4及增重效果优于油乳剂和铝胶佐剂.结论:综合评价以蜂胶佐剂效果最好,能增强猪细小病毒灭活疫苗的免疫效果,是一种良好的疫苗佐剂.更多还原

【Abstract】 This work evaluated the adjuvant activity of propolis,oilemulsion and aluminium salts(alum) associated to inactivated porcine parvovirus(PPV) vaccine.To carry out immune response test,blood samples were collected for measuring antibody titer, spleen peripheral lymphocyte proliferation were determined after vaccination,supernatants of T cell cultures were used for cytokines(IL-2 and IL-4) determination.The result showed that three adjuvants can improve the immune effect of PPV vaccine. Oilemulsion and propolis adjuvants presented higher levels of antibodies compared to alumimum salts adjuvant.The use of PPV vaccine with propolis was able to increase the cellular immune response,it significantly increasing T lymphocyte proliferation, inducing IL-2(Th1 cytokine),IL-4(Th2 cytokine) production,promoting weight gain than that of using oilemulsion and alum adjuvants to PPV vaccine,The effect of propolis on the humoral and cellular immune responses may be exploited for the development of effective vaccines.更多还原