【作者】 周玉兰； 王鲜忠； 周银涛； 赵伯川； 陈永军； 张家骅；

【机构】 西南大学动物科技学院重庆市牧草与草食家畜重点实验室；

【摘要】 氯氰菊酯(Cypermethrin,CYP)对于生殖系统具有损害作用,但对睾丸支持细胞的影响仍不清楚。本研究以β-氯氰菊酯为试验药物,用VE作为抗氧化剂,以成年公鼠(30-35g)为实验材料,通过灌喂方式给药,处理35d,通过免疫组化检测睾丸中PCNA、CX43的表达,并检测小鼠睾丸的总抗氧化能力的变化。结果发现,β-氯氰菊酯明显增加了睾丸支持细胞中PCNA的表达(P<0.05),减少了CX43的表达,降低了睾丸总抗氧化能力(P<0.05);加入VE后,睾丸支持细胞中PCNA的表达减少;CX43的表达量增加;睾丸总的抗氧化能力增强(P<0.05)。这表明,β-CP能够通过氧化应激使支持细胞去分化,从而抑制精子的生成,造成精子生成能力下降。更多还原

【Abstract】 Cypermethrin has deleterious effects on male reproductive function.The objective of this study was to identify whether the effects of beta-CYP on Sertoli cells Dedifferentiation were associated with oxidative stress.A dose of beta-CYP (20 mg/kg) was administered to male mice for 35 d with or without vitamin E(20 mg/kg).The expression and distribution of PCNA,CX 43 in testes were detected by immunohistochemistry and the testis total antioxidant capacities were detected. Beta-cypermethrin not only increaed the expression of PCNA in Sertoli cells,but also decreased the expression of Cx43 in Sertoli cells and total antioxidant capacity(TAC)(P<0.05).Vitamin E reversed the effects of beta-CYP on he expression of PCNA,Cx43 in Sertoli cells and total antioxidant capacity(P<0.05).Therefore,beta-CYP could causes Sertoli cells dedifferentiation by inducing oxidative stress.更多还原