【作者】 潘利华； 陆俊； 罗建平；

【Author】 Lihua Pan Jun Lu Jianping Luo (School of Biotechnology and Food Engineering,Hefei University of Technology,Hefei 230009)

【机构】 合肥工业大学生物与食品工程学院；

【摘要】 霍山石斛是一种可食植物,作为功能饮品在我国已有近2000年的食用历史,多糖是它的主要活性成分。本实验旨在研究霍山石斛多糖对亚硒酸钠致大鼠肝损伤和肝纤维化的干预效果。血清指标和肝组织病理切片分析显示,霍山石斛多糖可以明显改善亚硒酸钠导致的大鼠体重减轻、肝脏缩小和脾脏增大的症状,有效干预肝损伤和肝纤维化的发展,对亚硒酸钠导致的大鼠血清ALT、AST和LDH活性升高具有显著的抑制作用。肝组织氧化状态、相关抗氧化酶活性以及肝实质细胞膜流动性分析表明,霍山石斛多糖可以恢复亚硒酸钠处理的大鼠肝脏内H₂O₂、GSH、MDA、sOD、CAT和GST至对照的水平,维持肝实质细胞膜的正常流动性。免疫荧光组织化学分析证明,霍山石斛多糖对肝损伤和肝纤维化的干预和它有效减少转化生长因子TGF-β1和肝脏内胶原蛋白表达相关。研究结果表明,霍山石斛多糖对肝损伤和肝纤维化的干预活性,使它有可能发展成为一种新的预防肝脏疾病的药物和保健食品。更多还原

【Abstract】 This study was carried out to investigate the preventive effects of a homogeneous polysaccharide from Dendrobium huoshanense on liver injury and fibrosis induced by sodium selenite.Sprague-Dawley rats with the subcutaneous injection of sodium selenite at the dosage of 3.28 mg kg^-1 b.wt.were set as the animal model.Rats treated with sodium selenite and polysaccharide at 50（low dose ）,100（medium dose）, and 200（high dose ）mg kg^-1 b.wt.were used as the prevention groups.Biochemical and histological analysis showed that the polysaccharide significantly ameliorated liver injury and prevented the progression of liver fibrosis.Oral administration of polysaccharide effectively attenuated the toxicity of selenite to liver tissue judged by the decreased activities of serum aminotransferase（ALT,AST and LDH）.Meanwhile, polysaccharide also reduced the levels of H₂O₂ and malondialdehyde（MDA）,elevated the levels of GSH, restored the fluidity of hepatic cytoplasm membrane,and retained the activities of endogenous antioxidative enzymes SOD,CAT and GST.Furthermore,the prevention of liver injury and fibrosis by the polysaccharide was supported by reduced expression of TGF-β1 and type I collagen.These results suggested that the polysaccharide may be developed into a novel antifibrotic agent for the prevention of liver injury and fibrosis.更多还原