【作者】 王健； 孙颖； 王丽英； 朱晓楠； 毕宁； 林凡； 宋大千；

【Author】 WANG Jian~1,SUN Ying~1,WANG Li-ying~1,ZHU Xiao-nan~1,BI Ning~1,LIN Fan~2 and SONG Da-qian~(*1)(College of Chemistry,Jilin University,Changchun 130012)

【机构】 吉林大学化学学院； 东北师范大学生命科学学院；

【摘要】 在金膜上修饰了三层二氧化钛溶胶凝胶。为了提高灵敏度,金纳米粒子被固定在第二层溶胶凝胶中。在第三层溶胶凝胶盅,金纳米粒子耦合的抗体分子被包埋,由于金纳米粒子可以提供生物兼容的微环境,这样就较好的保持了抗体的活性,从而增强了传感器的稳定性,并提高了灵敏度。更多还原

【Abstract】 A novel surface plasmon resonance(SPR) biosensor for the determination of human IgG was introduced. The biosensor was prepared with three layers of titania sol-gel membrane by vapor deposition.The colloid Au nanoparticle (AuNP) was immobilized in the second layer of titania membrane and the AuNP coupled with rabbit anti-human IgG was encapsulated in the third layer.The AuNP in the second layer of titania membrane was proved to be effective in the sensitivity enhancement of SPR biosensor.The titania sol-gel was also confirmed to be benefit for biomolecule to keep bioactivity,which could offer a good waterish microenvironment.更多还原