【作者】 储艳秋； 蒋公羽； 姜丹； 王青； 丁传凡；

【Author】 CHU Yan-qiu,JIANG Gong-yu,JIANG Dan,WANG Qing and DING Chuan-fan~*(Laser Chemistry Institute, Chemistry Department,Fudan University,Shanghai 200433)

【机构】 复旦大学化学系激光化学研究所；

【摘要】 <正>在过去的十多年里,质谱新技术和新方法取得了快速的进展,其中串级质谱已经成为一个重要的工具被广泛地应用于有机化合物分子结构分析和多肽分子中的氨基酸序列测定。近年来,有关非共价复合物的碎片化过程的质谱研究也开更多还原

【Abstract】 The secondary tandem mass spectrometry（MS²） results indicated that collision gas thickness（CGT） and collision energy are two important conditions affecting the fragmentation pathway of glutathione complex.When the collision gas thickness maintained at 50×10¹³ molecules/cm².With an increase of collision energy from 5 eV to 40 eV, the intensity of mass spectrum for the precursor His-GSH complex decreased gradually.After that,the intensity for the precursor increased gradually.When the collision energy fixed at 30 eV and CGT rise up to 75×10¹³ molecules/cm²- the covalent bond also began to dissociate,leading to the formation of y₂ and b₂ for glutathione.To confirm the fragment ion y₂ of glutathione,MS³ was also performed and y₁ was detected.更多还原