【作者】 胡建鹏； 周会； 王键； 王丽娜； 吴生兵； 孟庆萍；

【Author】 HU Jiang-peng,ZHOU Hui,WANG Jian~△,WANG Li-na,WU Sheng-bing,MENG Qing-ping (Anhui College of Traditional Chinese Medicine,Hefei 230038,China)

【机构】 安徽中医学院；

【摘要】 目的探讨益气活血方和补肾生髓方对脑缺血再灌注大鼠缺血半暗带Notch-1和Jagged1蛋白表达的影响。方法线栓法制作局灶性脑缺血再灌注大鼠模型。随机分为假手术组、模型组、益气活血方组和补肾生髓方组。脑缺血2h,分别再灌注1w、2w、3w,采用免疫组化SABC法检测Notch-1和Jagged1蛋白表达的阳性总面积和平均吸收光密度。结果在缺血侧额顶叶皮质缺血半暗带,再灌注各时间点,模型组Notch-1和Jagged1蛋白表达的阳性总面积和平均吸收光密度显著高于假手术组(P<0.05或P<0.01),两种中药复方组再灌注各时间点Notch-1和Jagged1蛋白表达的阳性总面积和(或)平均吸收光密度显著低于模型组(p<0.05或P<0.01)。结论:两种复方能通过降低缺血半暗带Notch-1、Jagged1蛋白表达,抑制Notch信号转导通路,影响局灶性脑缺血后神经干细胞增殖分化。更多还原

【Abstract】 AIM:To investigate the effects of Yiqihuoxue prescription and Bushenshengsui prescription on the expression of Notch-1 and Jagged1 protein in ischemic penumbra after local cerebral ischemia/reperfusion in MACO rat.METHODS:The local cerebral ischemia/reperfusion model was established by intraluminal thread occlusion of the middle cerebral artery(MCAO),The animals were randomly divided into pseudo suegery group(sham group),model group,Yiqihuoxue and Bushenshengsui prescription group.Using the techniques of immuno-histochemical staining,the expression of Notch-1 and Jagged1 was observed at 1,2 and 3 weeks in ischemic penumbra of the cortex of frontal and parietal lobe.RESULT:A value and positive unit of Notch-1 and Jagged 1 protein expression in control group were higher than those in sham in ischemic penumbra of the cortex of frontal or parietal lobe after local cerebral ischemia/reperfusion in MCAO rats(P<0.05 or P<0.01 ).A value and/or positive unit of their expression in Yiqihuoxue prescription and Bushenshengsui prescription group were lower than those in control group (P<0.05 or P<0.01).CONCLUSION:Yiqihuoxue prescription and Bushenshengsui prescription could Affect the proliferation and differentiation of neural stem cells,depress Notch signal transduction acess after local cerebral ischemia/ reperfusion in MCAO rats by inhibiting the expression of Notch-1and Jagged1 protein.更多还原