【作者】 林妮； 吕俊华； 潘竞锵； 肖柳英；

【Author】 LIN Ni1 Lü Jün-Hua2 PAN Jing-qiang3 XIAO Liu-ying3 (1 Guangzhou Hospital Traditional Chinese medicine, Guangzhou 510130, China; 2 Department of pharmacology, Jinan University, Guangzhou 510632, China; 3 Guangzhou institute of traditional Chinese Medicine and Materia Medica,Guangzhou 510130,China. )

【机构】 广州市中医医院； 暨南大学药学院药理研究室； 广州市中医医院-广州市中医中药研究所；

【摘要】 目的:探讨荔枝核提取物对乳腺增生病(HMG)大鼠乳腺组织雌二醇受体(ER)和孕酮受体(PR)表达及细胞凋亡作用。方法:取健康、未孕雌性SD大鼠60只,按体重随机抽取10只作为空白对照组,每天肌肉注射(im)生理盐水;其余50只大鼠im苯甲酸雌二醇0.5mg·kg-1qd×25d,改用黄体酮4mg·kg-1qd×5d,造成大鼠乳腺增生病模型;第31d按体重随机分组并给药,荔枝核高、中、低剂量(44g·kg-1,22g·kg-1和11g·kg-1)和乳结平(3.3g·kg-1)分别灌胃给予药物,正常对照组和模型组动物则给予蒸馏水,每天一次,连续给药30d;实验结束时,用免疫组化方法观察正常和HMG大鼠乳腺组织ER和PR表达,流式细胞仪检测乳腺组织细胞凋亡情况,以及计算子宫指数改变。结果:荔枝核高、中剂量和乳结平能抑制HMG大鼠乳腺组织ER和PR表达(P<0.01~P<0.05),荔枝核高、中剂量呈量效关系地促进乳腺组织细胞凋亡(P<0.01~P<0.05);HMG大鼠乳头红肿及增生明显,子宫指数增加,与空白对照组比较,差异有高度显著性(P<0.01),而各给药处理组能不同程度地逆转上述病理性改变(P<0.05~0.01)。结论:荔枝核能抑制HMG大鼠乳腺组织ER和PR表达,并促进乳腺组织细胞凋亡,改善乳腺增生及子宫的病理变化,从而有效地对抗雌激素性大鼠乳腺增生病。更多还原

【Abstract】 Objective: To study the effects of Litchi Seed extract (LSE) on mammary tissue for investigating expression of estradiol and progestogen receptor and apoptosis in rat with hyperplasia of mammary glands (HMG), and to explore its mechanism. Methods: To establish the rat pathologic model of HMG was replicated by intramuscular (im) ministration of estradiol benzoate 0.5mg·kg-1 qd for 25ds, subsequently progesterone 4mg·kg-1 for 5ds, as well as 10 in the blank control group im ministration of normal saline; affter 31 days, 50 rats were randomly divided into 5 groups according to body weight : there were 10 rats in HMG group, 30 in experimental drugs group which were given by LSE 44 g·kg-1, 22g·kg-1 and 11g·kg-1, 10 in positive control group by the administration of Rujieping (3.3g·kg-1), as well as 10 in the blank control group; then all drugs or solvent were adiministered respectively by intragastric administration (ig) qd for 30 ds. The end of the experiment, the expression of estradiol and progestogen receptor and apoptosis in nipples were measured by immunohistochemical method or flow cytometry, the height and diameter of nipples and indexes of uteri were observed between normal and model group in which negative control, all three dose groups (low, medial and high dose group) of LSE and Rujieping were treatd respectively. Results: Compared with model of HMG group, the height and diameter of nipples and indexes of uteri were diminished and the expression of estradiol and progestogen receptor were down-regulated and the apoptosis were accelerated by medial and high dose groups of LSE and Rujieping (P<0.05~0.01). Conclusion: Litchi Seed may down-regulate the expression of estradiol and progestogen receptor and accelerate the apoptosis in mammary tissue and inhibiti pathomorphological changes in breast tissue and uteri in the HMG rats.更多还原