【作者】 喻乾明； 季莉莉； 施松善； 秦坤； 王顺春； 王峥涛；

【Author】 YU Qian-ming~1,JI Li-li~(1,2*),SHI song-shan~1,SHEN Kai-kai~1,QIN Kun~1,WANG Shun-chun~(1,2*),WANG zhen-tao~(1,2) (1.The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines,Institute of Chinese,Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine,Shanghai 201210,China;2.Shanghai R&D Center for Standardization of Chinese Medicines, Shanghai 201210,China)

【机构】 上海中医药大学中药研究所中药标准化教育部重点实验室中药新资源与质量标准综合评价国家中医药管理局重点研究室； 上海中药标准化研究中心；

【摘要】 目的探讨蜈蚣藻多糖（GFP）的抗肿瘤作用。方法观察GFP对小鼠S-180肉瘤生长的影响;应用MTT比色法测定GFP对HUVEC细胞存活率的影响;用Millicell小室测定GFP对HUVEC细胞迁移能力的影响;用ECMatix胶测定GFP对HUVEC细胞管腔形成的影响。结果80mg.kg^-1GFP明显抑制S-180肉瘤的生长作用,抑制率为51.9%;GFP在5、10、20、50、100mg.L^-1浓度下处理HUVEC细胞48h,细胞存活率没有显著性的变化;在50、100 mg.L^-1时能显著性地抑制HUVEC细胞的迁移并呈剂量依赖关系;在100 mg.L^-1时能显著性地抑制HUVEC细胞的管腔形成。结论GFP能够抑制小鼠S-180肉瘤的生长,其抗肿瘤作用可能与抑制肿瘤血管生成有关。更多还原

【Abstract】 OBJECTIVE The present study was designed to observe the anti-tumor properties of Grateloupia filicina polysaccharide（GFP）.METHODS The anti-tumor properties of GFP was assessed in mice bearing S-180 sarcoma cells;The survival rate of HUVEC was assessed using MTT method after treating with GFP.The effect of GFP on HUVEC migration ability was observed using Millicell.The effect of GFP on HUVEC tube formation was assessed using ECMatix.RESULTS: 80mg.kg^-1GFP inhibits tumor growth in mice bearing S-180 sarcoma cells and the inhibit rare was 51.9%;The HUVEC cell viability did not change significantly after being treated with GFP for 48h at dose of 5、10、20、50、100 mg.L^-1.The migration of HUVEC was significantly inhibited at dose of 50、100 mg.L^-1and it was dose-dependently blocked by GFP.GFP disrupted tube formation on HUVEC cells in Matrigel at a dose of 100 mg.L^-1.CONCLUSION GFP can inhibit tumor growth and may associated with inhibition ofangiogensis.更多还原