【作者】 朱翔杰； 周冰峰； 吴显达； 徐新建；

【Author】 Zhu Xiangjie,Zhou Bingfeng,Wu Xianda,Xu Xinjian (College of Bee Science,Fujian Agriculture and Forestry University,Fuzhou 350002)

【机构】 福建农林大学蜂学学院；

【摘要】 通过采集自温州大门岛及邻近大陆,共133群中华蜜蜂样本,进行30个形态遗传标记、5个微卫星DNA位点及线粒体DNA tRNA^leu～COⅡ序列分子遗传标记分析,研究中华蜜蜂种群遗传结构及遗传分化,探索中华蜜蜂岛屿种群形成机制。研究结果发现岛屿与大陆的海域隔离最小直线距离约7 km的条件下,形态、微卫星DNA和线粒体DNA等遗传标记数据分析表明,岛屿中华蜜蜂与大陆中华蜜蜂表现出明显的种群遗传分化。具体表现在岛屿中华蜜蜂具有较小翅长,较小的蜡镜和蜡镜间距,较小翅脉角O26、N23,较大翅脉角J10、E9（P<0.05）,线粒体DNA单倍型多样性低。微卫星数据显示分化系数大于0.1。更多还原

【Abstract】 Population genetic structure and genetic differentiation of Apis cerana cerana adult workers from one hundred and thirty three colonies collected from Damen island and adjacent land were studied by thirty morphometric characters and polymorphism of five microsatellite DNA markers and tRNA^leu～COII sequence of mitochondrial DNA.The objectives of this study were to explore the mechanism of island population formation.The results showed that there were significant genetic differentiation between island and adjacent land honeybee populations based on morphometric analysis,microsatellite DNA and mitochondrial DNA data.Under the 7 km minimum straight-line distance between island and adjacent land,honeybee of island population had shorter wing,smaller wax plate and distance between wax plate,smaller wing angle O26 and wing angle N23,larger wing angle J10 and wing angle E9（P<0.05）,less mitochondrial haplotypes, simple genetic structure.The F_ST values were larger than 0.1.更多还原