【作者】 张俊清； 陈文芝； 蒋德锡； 张丹；

【Author】 ZHANG Jun-qing~1,CHEN Wen-zhi~2,JIANG De-xi~2,ZHANG Dan~(2*) (1.West China pharmacy school of Si Chuan University,Chengdu 610041;2.Affiliated Hospital of Luzhou Medical College,Luzhou 646000,China)

【机构】 海南医学院药学系； 四川大学华西药学院；

【摘要】 目的建立同时测定角花胡颓子中齐墩果酸与熊果酸含量的RP-HPLC法。方法采用Diamonsil C₁₈（250mm×4.6mm,5μm）,以甲醇-0.5%乙酸铵（85:15）为流动相,检测波长为206nm,流速为1.0ml·min^-1,柱温30℃。结果齐墩果酸进样量在0.06～0.36μg（r=0.9995）、熊果酸进样量在0.65～3.9μg（r=0.9997）范围内,线性关系良好,齐墩果酸与熊果酸的平均加样回收率分别为99.16%和102.46%,RSD分别为8.35%和6.00%（n=9）,重复性试验的含量RSD分别为6.91%和4.24%（n=6）。结论该法简便、快速、准确,可用于角花胡颓子的质量控制。更多还原

【Abstract】 OBJECTIVE To establish an RP-HPLC for determination of oleanolic acid and ursolic acid in Elaeagnus gonyanthes Benth.METHODS The analytical column was Diamonsil C₁₈ column（250mm×4.5mm,5μm）.The mobile phase consisted of methanol- 0.5%ammonium acetate（85:15）.The detective wavelength was 206nm.The flow rate was 1.0 ml·min^-1 and the column temperature was 30 degrees.RESULTS Oleanolic acid and ursolic acid showed a good linearity in range of 0.06～0.36μg（r = 0. 9995） and 0.65～3.9μg（r= 0.9997）.The average recovery of oleanolic acid was 99.16%with relative standard deviation（RSD ） of 8.35%and the ursolic acid was 102.46%with RSD of 6.00%（n = 9）,the reproducibility test of content was 6.91%and 4.24% （n = 6）.CONCLUSION This method is simple,quick,accurate and can be used for quality control of Elaeagnus gonyanthes Benth.更多还原