【作者】 和凡； 钟国平； 赵立子； 毕惠嫦； 黄民；

【Author】 HE Fan1, 2, ZHONG Guo-ping2, ZHAO Li-zi2, BI Hui-chang2, HUANG Min2 (1. Department of Pharmacy, Guangzhou Women and Children’s Medical Center, Guangzhou 510120, China; 2. School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510080, China)

【机构】 广州市妇女儿童医疗中心； 中山大学药学院；

【摘要】 目的研究丹参酮IIA对大鼠细胞色素P450酶的诱导作用。方法丹参酮IIA7,20,60,180mg/(kg·d)4个剂量组,雄性SD大鼠连续ig7d,取肝脏组织,应用体外"cocktail"方法,反转录-聚合酶链反应,免疫印迹杂交方法分别检测CYP450酶活性,基因表达和蛋白表达的变化。结果丹参酮IIA能显著诱导CYP1A2,CYP2E1的活性以及基因、蛋白表达,且呈剂量依赖性;高剂量组能增强CYP2C19的活性,但对基因、蛋白表达没有影响;对包括CYP3A1在内的其他亚型没有影响。结论丹参酮IIA对大鼠多种CYP450亚型有诱导作用。更多还原

【Abstract】 Objective To study the induction of cytochrome P450 enzymes by tanshinoneIIA in rats. Methods Male SD rats were administered daily dose of tanshinoneIIA (7, 20, 60, 180 mg·kg-1), ig, for 7 consecutive days. The levels of CYP450 activity, mRNA, protein in rats liver were analyzed by in vitro cocktail, RT-PCR, Western blot, respectively. . Results TanshinoneIIA induced significantly the activity, protein, and mRNA expression of CYP1A2 and 2E1 in a dose-dependent manner; High dose also increased CYP2C19 activity; there was no effect on other CYP isoforms. Conclusion TanshinoneIIA can induce several CYP isoforms in rats,.更多还原