【作者】 梅雪婷； 许东晖； 罗小菊； 许实波；

【Author】 MEI Xue-ting, LUO Xiao-ju, XU Dong-hui (Lab. of Chinese Medicine and Marine Drugs, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275)

【机构】 中山大学生命科学学院中药与海洋药物实验室；

【摘要】 本论文利用沙田柚、茶叶提取物制备绿色天然食品绿原知己茶橘片,通过建立小鼠急性酒精性肝损伤、大鼠慢性酒精性肝损伤动物模型,考察茶橘片对急慢性酒精性肝损伤的保护作用。50%乙醇(0.1mL/10g)灌胃小鼠,每天给予两次,共10d,可成功建立小鼠急性酒精性肝损伤模型。结果表明:与模型组比较,茶橘片(100、300、900mg/kg)剂量能显著降低血清TG含量,减少肝组织MDA的生成,提高GSH-ST的活性,呈剂量依赖关系(P<0.001)。采用酒精灌胃(50%乙醇,4g/kg),喂养高脂饲料,腹腔注射微量CCl4(25%CCl4-花生油溶液,0.1mL/kg)的方法八周可成功建立慢性酒精性肝损伤大鼠模型。与模型组比较,茶橘片(70、210、630mg/kg)可显著降低血清ALT水平,增加血清ALB含量,减少肝组织MDA及CHO的生成,增加SOD的活性,呈剂量依赖关系(P<0.001)。茶橘片由于富含天然黄酮类活性物质能够减轻酒精对脂肪代谢的影响,对急慢性酒精性肝损伤起保护作用。更多还原

【Abstract】 Tea-Orange Tablets of Greenfield Zhiji were made from extracts of Satian Yu and tea. Acute and chronic alcoholic hepatic injury animal models were setup to test the protective effects of Tea-Orange Tablets. Oral administration 50% alcohol twice a day for 10 days setup mice acute alcoholic hepatic injury models. Compared to control group, Tea-Orange Tablets (100、300 and 900 mg/kg)could reduced serum TG and MDA contect, improve activity of GSH-Px with dose dependence (p<0.001). Chronic alcoholic hepatic injury rat models was setup by 50% alcohol (4 g/kg, p.o.) and 25% CCl4 (0.1 mL/kg, i.p.) for 8 weeks. Oral administration Tea-Orange Tablets (70、210 and 630 mg/kg) could significantly reduce content of ALT, MDA and CHO, improve content of ALB and activity of SOD, with dose dependence (p<0.001). Tea-Orange Tablets rich with flavones material had protective effects to acute and chronic alcoholic hepatic injury.更多还原