【作者】 王建宇； 林海； 孙林丽； 但卫华； 但年华； 胡杨； 汤圣；

【Author】 WANG Jian-yu~(1,2),LIN Hai~(1,2),SUN Lin-li~1,DAN Wei-hua~(1,2), DAN Nian-hua~(1,2),HU Yang~(1,2),TANG Sheng~3 (1.Department of Biomass Chemistry and Engineering,Sichuan University,Chengdu 610065,China; 2.The Research Center of Biomedical Engineering,Sichuan University,Chengdu 610065,China; 3.Jiangyin Benshine Biotechnology Co.,Ltd.,Jiangyin 214432,China)

【机构】 四川大学生物质化学与工程系； 四川大学生物医学工程研究中心； 江阴奔翔生物科技股份有限责任公司；

【摘要】 将聚乙烯醇改性引入羧基,在1-(3-二甲氨基丙基)-3-乙基碳二亚胺盐酸盐(EDC)和N-羟基琥珀酰亚胺(NHS)的作用下使其与胶原共价交联。采用不同工艺条件制备水凝胶,如改性PVA与胶原的质量比,交联剂的用量以及冻融次数等。运用Design Expert软件,采用响应曲面法分析水凝胶的机械性能(拉伸强度和断裂伸长率),并建立数学模型。结果表明,响应面模型能对响应值进行拟合且拟合程度较高。此外,实验结果还表明因素之间的相互作用比单因素对聚乙烯醇-胶原水凝胶的机械性能有更明显的影响。通过对变化趋势的分析,认为影响它们的主要原因可能是水凝胶重定位或重结晶后的结构。更多还原

【Abstract】 The poly(vinyl alcohol) was modified to introduce the carboxyl groups which can be utilized to covalent bond with collagen through the cross linke,that is 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-Hydroxysuccinimide.The hydrogels were prepared with different techniques,such as the ratio of modified PVA to collagen,the cross-linker and the times of freezing and thawing.Using a response surface methodology,the mechanical properties of the hydrogels(tensile strength and elongation at break) were analyzed. The results indicated that the response surface models could significantly fit the response values.Moreover, the results shown that the interactions between factors had more obvious effects to the physical properties of PVA-COL hydrogels than the single one.After the analysis of factors,influences and deduced from the data and trends,the driving force behind these might be the re-orientation or re-crystallization structure of the hydrogels.更多还原