【作者】 武松； 潘嘉云； 王学忠； 张国庆； 蒋伟康； 汤林华；

【Author】 WU Song~(1,3),PAN Jia-yun~1,WANG Xue-zhong~2,ZHANG Guo-qing~1 JIANG Wei-kang~1,TANG Lin-hua~1 (1.National Institute of Parasitic Diseases Chinese Center for Disease Control and Prevention,Shanghai 200025,China; 2.Yunnan Institute of Parasitic Diseases,Puer 665000,China; 3.School of Integrated Traditional and Western Medicine of Anhui College of Chinese Traditional Medicine,Hefei 230038,China)

【机构】 中国疾病控制中心寄生虫病预防控制所； 世界卫生组织疟疾、血吸虫病和丝虫病合作中心； 安徽中医学院中西医结合临床学院； 云南省寄生虫病研究所；

【摘要】 目的确定西藏疟疾流行区墨脱县的疟疾传播媒介。方法根据2005、2006和2007年疟疾发病情况,在西藏林芝地区墨脱县选择了3个疟疾发病较高的自然村,采用人诱、牛诱、灯诱及清晨人房全捕等方法捕获成蚊,经形态学鉴定后麻醉处死,密封干燥,带回实验室-20℃冻存备用,采用混合样本法随机抽取10只多斑按蚊复合体为一份混合标本,提取蚊虫DNA。根据文献采用巢氏PCR扩增疟原虫小亚单位核糖体脱氧核糖核酸(SSurDNA),并对阳性结果克隆测序,网络比对证实。结果共捕获按蚊5 345只,其中多斑按蚊复合体5 190只,带足按蚊155只,提取的360份多斑按蚊复合体混合样本DNA中发现子孢子阳性扩增样品2份,种型鉴定2份阳性混合样本均为伪威氏按蚊,PCR产物经克隆测序,NCBI BLAST同源性比对证实为疟原虫SSu rDNA基因片段。结论多斑按蚊复合体中的伪威氏按蚊为西藏林芝疟疾流行区的传疟媒介。更多还原

【Abstract】 Objective To find the malaria vector in malaria epidemic area in Linzhi district,Tibet.Methods Three villages with higher malaria incidence were selected as investigation spot.Human-baited traps,cow-baited traps,CDC light traps and morning collecting were adopted to collect mosquitoes.Mosquitoes were killed after identified as Anopheles macidatus complex and stored with desiccant and at -20℃.DNA extracted with mixed mosquitoes sample. SSu rDNA of sporozoites was amplified according to reference,and positive products of PCR were cloned,sequenced and blasted for confirm.Results Among total 5345 anopheles mosquitoes, Anopheles macidatus complex take for 5190,and the remainders were An.peditiaeniatus.Two positive samples were found in 360 mixed Anopheles maculatus complex DNA samples,and were identified as Anopheles psewiUmori with species identification method Conclusion Anopheles psewiUmori of Anopheles maculatus complex was the malaria vector in Iinzhi malaria endemic area.更多还原