【作者】 孙雷； 张骊； 黄耀凌； 汪霞； 王树槐；

【Author】 SUN Lei,ZHANG Li,HUANG Yao-ling,WANG Xia,WANG Shu-huai (China Institute of Veterinary Drug Control,Beijing 100081,China)

【机构】 中国兽医药品监察所；

【摘要】 <正>链霉素(streptomycin)是从放线菌属灰链丝菌培养液中提取的一种氨基糖苷类抗生素,兽医临床上常用于预防和治疗革兰氏阴性菌和结核杆菌等引起的感染。由于其抗菌谱广,而且价格低廉,因此在奶牛养殖业中常被大量应用于治疗奶牛乳腺炎等。链霉素使用后可对肾脏和耳听神经造成不可逆转的更多还原

【Abstract】 A LC - MS/MS method was established for the determination of streptomycin and dihydrostreptomycin in milk.The chromatographic analysis was performed on BEH C₁₈ column of 50 mm×2.1 mm,1.7μm with a mobile phase consisting of 20 mmol/L HFBA acetonitrile and 20 mmol/L HFBA as at flow rate of 0.3 mL/min under 30℃.Mass spectrometry was run under positive ESI and MRM mode.The calibration curve showed a good linearity between the peak areas and the concentrations from 0.05 to 2 mg/L with R² more than 0.998.The of detection limit of the method was 2.5μg/kg,and the limit of quantification was 5μg/kg.The average recoveries from spiked milk at the three concentration levels of 50,100 and 200μg/kg ranged from 83%-113%.The intra-and inter-batch variation coefficients were range of 1.8%-8.9%and 2.4%- 12.2%,respectively.更多还原