【作者】 梁芳； 孙宪芝； 王文莉； 孙霞； 郑成淑；

【Author】 LIANG Fang SUN Xian-zhi WANG Wen-li SUN Xia ZHENG Cheng-shu (College of Horticulture Science and Engineering,Shandong Agricultural University; State Key Laboratory of Crop Biology,Tai ’an 271018)

【机构】 山东农业大学园艺科学与工程学院作物生物学国家重点实验室；

【摘要】 以切花菊品种‘神马’为材料,研究了低温弱光胁迫下乙酰水杨酸（ASA）和CaCl₂处理对菊花光合机构及活性氧清除酶系统的影响,探讨了ASA和CaCl₂缓解菊花对低温弱光胁迫效应的可能的生理机制。结果表明:低温弱光胁迫下,与对照相比,ASA和CaCl₂处理抑制了净光合速率（Pn）、羧化效率（CE）、表观量子效率（AQY）、暗适应下最大光化学效率（Fv/Fm）以及实际光化学效率（ΦPSⅡ）和光化学猝灭（qP）的大幅度降低,而且明显提高了叶绿素含量,相对提高了叶绿体数和淀粉粒数,使菊花叶片细胞体积增大。表明ASA和CaCl₂处理可有效调控低温弱光下菊花叶片的光合性能,提高了对低温弱光的耐性;低温弱光胁迫下,与对照相比,ASA和CaCl₂处理的菊花叶片相对电导率及膜透性伤害率显著下降,并提高了SOD、POD和CAT活性。说明ASA和CaCl₂处理对低温弱光胁迫条件下菊花叶片保护酶活性具有正调控作用,这种正调控作用可能对低温弱光条件下菊花叶片的光合系统起到保护作用。综合作用效果表明,ASA与CaCl₂复配处理优于ASA或CaCl₂单独处理。更多还原

【Abstract】 Taking cut flower chrysanthemum ’ Shenma’ as testmaterial,the effects of acetylsalicylic acid（ASA） and CaCl₂ treatments on its photosynthetic system and antioxidant enzyme activities under the stress of low temperature and poor light were investigated,and the possible physiological mechanisms of ASA and CaCl₂ was discussed.The results showed that under the stress of low temperature and poor light,the treatments of ASA and CaCl₂ addition greatly inhibited the netphotosynthesis rate （Pn）,carboxylation efficiency（CE）,apparent quantum yield（AQY）,maximal photochemical efficiency of PSⅡin the dark （Fv/ Fm）,quantum yield of PSⅡelectron transport（ΦPSⅡ） and photochemical quenching（qP） compared with those of control,single application of ASA or CaCl₂.The combination application of ASA and CaCl₂ obviously improved the chorophyll content,relatively improved the number and size of chloroplasts and starch grainins in the leaves of chrysanthemum under the stress of low temperature and poor light.The results suggested that ASA and CaCl₂ could regulate the leaf photosynthetic functions in the leaves of chrysanthemum,and enhanced the resistance against low temperature and light intensity.ASA and CaCl₂ could reduce relative conductance rate and injury rate in leaves of chrysanthemum seedlings and increase the leaf SOD,POD and CAT activities and soluble protein content compared with the control,indicating that ASA and CaCl₂ had positive regulation effect on the defense enzyme activities in chrysanthemum leaves,which could protect the photosynthetic system ofchrysanthemum under the the stress of low temperature and poor light to a certain degree.Induced resistance effect of ASA and CaCl₂ mixture was better than single ASA or CaCl₂更多还原