【作者】 王建辉； 刘建军； 陈克玲； 李洪雯； 何建； 关斌；

【Author】 WANG Jian-hui~1,LIU Jian-jun~(2*),CHEN Ke-ling~1,LI Hongwen~1,HE Jian~1,and GUAN Bin~1 (1 Horticulture Institute,Sichuan Academy of Agriculture and Science,Chengdu 610066,China;2 Sichuan Academy of Agriculture and Science,Chengdu 610066,China)

【机构】 四川省农业科学院园艺研究所； 四川省农业科学院；

【摘要】 葡萄是一种在全世界栽培面积大、产量高的重要果树。葡萄可以被多种病毒感染,分别诱导的不同症状,国内目前主要包括:卷叶病、皱木复合病和扇叶病。一株被4种病毒GLRaV-2,GLRaV-3,GVA和GVB复合感染的"藤稔"葡萄韧皮部为材料,分别回收RT-PCR产物并克隆得到4种病毒的全长外壳蛋白基因cp。利用DNAStar软件的ClustalW方法,分别对4种病毒的在NCBI上登陆的不同地理分离株的全长cp进行多重比对分析。根据每种病毒的不同分离株的全长cp多重比对同一性值大小,16个不同地理分布GLRaV-2分离株分成4个变异组;16个不同地理分布的GVA分离株分成2个变异组;7个不同地理分布的GVB分离株分成3个变异组。8个不同地理分布的GLRaV-3分离株的cp比对同一性高达99.9-92.5%,也许是一类单独、不分化的种群。进行葡萄病毒的结构蛋白基因系统进化研究,为以后利用病毒诱导基因沉默开展葡萄抗病育种打下理论基础。更多还原

【Abstract】 Grapevine is one of the most important economic horticulture crops with high yields around the world.So far there are more and more viruses that have been found to infect the grapevine with different symptoms,including leaf roll disease,rugose wood complex and fan leaf disease etc.One table grape variety, Fujiminori,was infected with four distinct of viruses using DAS-ELISA,including GLRaV-2,GLRaV-3, GVA and GVB,which had been confirmed by RT-PCR.Based on cp genes homology by multi-alignment analysis comparing with the known isolates of each virus collected from Genebank,it was seemed to cluster 16 cp genes of GLRaV-2 isolates into three distinct groups,to cluster 16 cp genes of GVA isolates into two distinguished groups,to cluster 7 cp genes of GVB isolates into three groups.The high identity of GLRaV-3 cp genes at the nucleotide sequence among total 8 isolates was 99.9-92.5%,it could conclude GLRaV-3 has a single undifferentiated population.The phylogenetic studies on cp of four main grapevine viruses could help grapevine virus-resistant breeding program using technique of virus induced gene silence in the future.更多还原