【作者】 王蓓； 吴灶和； 简纪常； 鲁义善； 汤菊芬； 黄郁葱；

【Author】 WANG Bei,WU Zao-he,JIAN Ji-chang,LU Yi-shan,TANG Ju-fen, Huang Yu-cong (Fisheries College of Guangdong Ocean University,Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals & Key Laboratory of Diseases Controingl for Aquatic Economic Animals of GuangDong Higher Education Institutions,Zhanjiang 524025,China)

【机构】 广东海洋大学水产学院,广东省水产经济动物病原生物学及流行病学重点试验室暨广东省高等学校水产经济动物病害控制重点实验室；

【摘要】 生物被膜存在广泛、难以发现并且具有处理困难等特点,对养殖业具有潜在的危害,并能引起水产品安全问题。本文以溶藻弧菌为研究对象,对定量检测生物被膜的超声波平板法进行条件优化,对优化的超声波平板法与平板擦拭法进行比较,并采用银染法进行验证。结果表明:优化的超声波平板法的最佳条件为:超声波功率120W,处理时间14min,处理温度25℃,优于平板擦拭法。更多还原

【Abstract】 Biofilms are widespread and hard to be detected and removed.The aquaculture production and fishery product safety are threatened by biofilms.Vibrio alginolyticus was used as a biofilms model strain in this stuty.The ultrasonic-plate method was optimized and compared with the plate-swabbing method,then the silver-staining method was used for verification.The results showed that the optimal conditions for ultrasonic-plate method were:ultrasonic power 120W,treatment time 14 min and treatment temperature 25℃.The optimized ultrasonic-plate method was superior to the plate-swabbing method.更多还原