Construction sandwich structure of bladder wall in vitro by tissue engineering approach

【作者】 卢慕峻； 周广东； 刘伟； 王忠； 曹谊林；

【Author】 Mujun Lu1, Guangdong Zhou2, Wei Liu2, Zhong Wang1, Yilin Cao2 1. Shanghai Jiaotong University, School of Medicine, 9th People’s Hospital, Department of Urology 2. Shanghai Jiaotong University, School of Medicine, 9th People’s Hospital, Tissue Engineering Key Laboratory 200072

【机构】 Shanghai Jiaotong University,School of Medicine,9th People’s Hospital,Department of Urology； Shanghai Jiaotong University,School of Medicine,9th People’s Hospital,Tissue Engineering Key Laboratory； Shanghai Jiaotong University,School of Medicine,9th People’s Hospital,Tissue Engineering Key Laboratory；

【摘要】 <正>Objectives: Using bladder acellular matrix graft (BAMG) as scaffold, urothelial cell (UC) and smooth muscle cell (SMC) as seeding cells, to form a sandwich structure of bladder wall in vitro. Materials and methods: Porcine bladders were harvested from adult pig and treated with sodium dodecyl sulfate, Triton X-100 and distilled water for decellularization. BAMG section were evaluated更多还原