【作者】 王小平； 孙理军； 李翠娟； 孙耀光； 林洁；

【Author】 WANG Xiao-ping,SUN Li-jun,LI Cui-juan,SUN Yao-guang,LIN Jie (Laboratory of Pathology,Shaanxi University of Chinese Medicine, Xianyang 712046,Shaanxi,China)

【机构】 陕西中医学院基础医学院；

【摘要】 为研究人肝癌组织中热休克蛋白70(HSP72)与甲胎蛋白(AFP)的相互作用,采用免疫化学、免疫荧光与共聚焦显微镜检测HSP72和AFP在肝癌细胞中的表达和定位。HSP72与AFP的相互关系通过免疫共沉淀和蛋白印迹杂交进行分析。结果免疫化学显示人肝癌细胞中存在高水平的HSP72和AFP共表达,均定位于细胞浆。AFP存在于HSP72单抗的免疫沉淀中,而HSP72则存在于AFP单抗的免疫沉淀中。结果表明人肝癌细胞HSP72与AFP相伴。两者之间的相互关系研究将成为探讨肝癌的发生和免疫治疗的新途径。更多还原

【Abstract】 To investigate the interaction between heat shock protein 72(HSP72) and alpha-fetoprotein(AFP) in human hepatocellular carcinoma cell.The expression and localization of HSP72 and AFP in human hepatocellular carcinoma cell were determined by immunocytochemistry, indirected immunofluorescence cytochemical staining and confocal laser microscopy.The interaction between HSP72 and AFP in hepatocarcinoma cells was analyzed by immunoprecipitation and Western Blot. Immunocytochemical staining detection showed hepatocellular cancer cells expressed high level of HSP72 and AFP synchronously.Both of them were stained in cell plasma.AFP existed in the immunoprecipitate of anti-HSP72 mAb,while there was HSP72 in the immunoprecipitate of anti-AFP mAb.The results suggest that HSP72 chaperones alpha-fetoprotein in human hepatocellular carcinoma cells.The interaction between HSP72 and AFP in human hepatocarcinoma cell can be a new route for studying the pathogenesis and immunotherapy of hepatocarcinoma.更多还原