【作者】 陈磊； 王霞； 富徐燕； 白岩； 金丹芳； 潘兰英； 田薇；

【Author】 Chen Lei~1,Wang Xia~2,Fu Xuyan~1,Bai Yan~1,Jin Danfang~1,Pan Lanying~1,Tian Wei~(1*) (1、Zhejiang Forest University forestry and biological technology institute,Zhejiang Linan 311300;2、Analysis Test Center of Shandong Province,Shandong,Jinan 250000)

【机构】 浙江林学院林业与生物技术学院； 山东省分析测试中心；

【摘要】 目的:建立了测定中药材白术中多菌灵农药残留量方法,并应用于白术中农药多菌灵的代谢分析。方法:采用RP-HPLC法,使用ODS不锈钢柱,用甲醇/水为流动相,检测波长为281 nm。结果:多菌灵在0.45～108ng的线性范围为,进样量与峰面积有良好的线性关系,最低检出量3.6×10^-10g,最低检测浓度为0.0225 ug·mL^-1。多菌灵在白术上最后一次施药到采收应不少于30 d。结论:本方法简便、快速、适合同类中药材中多菌灵农药残留量代谢测定,并为其安全监控提供实验依据。更多还原

【Abstract】 Objective:To establish a HPLC method for the determination of carbendazol in Chinese Traditional Medicine of atractylodes macrocephala.Method:The mobile phase constituted of methanol-water（35:65）.A C₁₈column was used,and carbendazol was determined at the detection wavelength of 281nm..Result:In the linear range of 0.45～108ng,the sample size and the peak area of carbendazol has a good linear relationship,the limit of detection is 3.6×10^-10 g,the minimum detectable concentration is 0.0225 ug / ml.Conclusion:This method is simple,rapid and reliable.It is suitable for the determination and safe control of carbendazol metabolism in this kind of Chinese Traditional Medicine, and providing experimental basis for its safety monitoring.更多还原