【作者】 张国梁； 林红； 戴敏； 陈益水；

【Author】 ZHANG Guo-liang,LIN Hong,DAI Min,CHEN Yi-shui. Department of Infectious Diseases,The first Hospital affliated to Anhui College of Traditional Chiese Medicine,Hefei 230031,China

【机构】 安徽中医学院第一附属医院感染科； 安徽中医学院中医内科； 安徽中医学院药学院；

【摘要】 目的研究中药软肝饮对CCl₄诱导的大鼠肝纤维化模型Smad3表达的影响,探讨其抗纤维化的作用机制。方法40只Wistar大鼠,随机分成正常对照组、模型组、软肝饮组和鳖甲软肝片组,采用CCl₄背部皮下注射构建肝纤维化模型,进行HE和Van-Gieson染色,光镜下观察肝组织纤维化程度。同时以免疫组织化学SABC法检测各组Smad3的表达。结果与正常对照组相比,模型组Smad3表达明显增强,软肝饮组大鼠肝脏Smad3的表达显著下调（0.279±0.085 vs 0.885±0.904,P<0.05）。另外,软肝饮组大鼠肝脏肝纤维化病理变化显著改善。结论软肝饮对CCl₄诱导的大鼠肝脏纤维化模型肝脏Smad3表达有明显的下调作用,可能是其抗肝纤维化主要作用机制之一。更多还原

【Abstract】 Objective To study the effects of ruanganyin on Smad3 expression of the experimental hepatic fibrosis induced by carbon tetrachloride（CCl₄） in rats,and to explore its anti-fibrotic molecular mechanism.Methods Forty healthy Wistar rats were randomly divided into normal control group,vacant control group,CCl₄-induced hepatic fibrosis group,and ruanganyin treatment group.Rats in normal group were treated with normal saline by intragastrical irrigation,and the other groups were subcutaneously injected with CCl₄ to establish hepatic fibrosis model.The histological changes of liver tissues were examined by pathological examination and protein expression of Smad3 was measured by immunohistochemical technique.Results Compared with normal control group,level of Smad3 in model group was elevated notably,but significantly decreased after treatment （0.279±0.085 vs 0.885±0.904,P<0.05）.The pathological changes of fibrosis liver tissues in ruanganyin group were also markedly alleviated.Conclusions Ruanganyin has significant inhibitory effects on CCl₄ induced hepatic fibrosis in rats,and the mechanism may be associated with the inhibition of Smad3 expression.更多还原