【作者】 张玉杰； 杨洁； 孙明杰； 刘丽梅； 邹晓翠； 孙万晶； 陈明霞；

【Author】 ZHANG Yu-jie,YANG Jie,ZOU Xiao-cui,SUN Wan-jing,CHEN Ming-xia (Beijing University of Chinese Medicine,Beijing 100102,China)

【机构】 北京中医药大学中药学院； 中国中医科学院基础理论研究所；

【摘要】 目的考察黄连总碱大鼠小肠吸收特性以及黄连肉桂配伍对黄连总碱小肠吸收的影响,探讨黄连肉桂药对配伍的规律。方法采用大鼠体外小肠吸收模型,考察时间、浓度及转运方向对黄连总碱大鼠小肠摄取和转运的影响;同时考察黄连与肉桂不同比例配伍后,黄连总碱的小肠吸收和转运的变化规律;采用薄层层析法对黄连肉桂水煎液、小肠浆膜液、组织匀浆液中的总碱成分进行定性鉴别。结果黄连水煎液中总碱成分具有与小檗碱类似的P-糖蛋白底物转运特性:总碱在大鼠小肠的摄取和转运具有时间及浓度依赖性,并且存在吸收饱和现象;总生物碱的转运明显受到转运方向的影响,从浆膜面到黏膜面的摄取和转运量明显大于从黏膜面到浆膜面;黄连与肉桂在一定配比范围内,黄连总碱的小肠吸收呈现明显增加的趋势,以黄连总碱为指标,二者配伍对黄连总碱的吸收存在最佳配比关系。黄连各生物碱成分经小肠吸收和转运特性无明显的差异。结论小肠吸收和体外化学反应共同决定了黄连肉桂药对的配伍作用。更多还原

【Abstract】 Objective:To research the principle of match-pair of huanglian-rougui.Methods:A rat intestinal model in vitro had been applied to investigate the absorption and transport characteristics of total huanglian alkali across intestinal mucosa,as well as the influence of match-pair of huanglian-rougui on its intestinal absorption.The identification of alkalis in primitive solution, serosal solution and intestinal homogenate were analyzed by TLC.Results:The transport and uptake characteristic of total alkali in huanglian solution was similar to berberine.The uptake and transport of total alkali across intestinal mucosa was concentrationand time-dependent,and showed a saturable process;Transport directions had strong effects on the transport and uptake of total alkali.The amount of total alkali transport from serosal side to mucosal side was much more than that from mucosal side to serosal side.After matching with rougui as some ration,the intestinal absorption of total alkali increased significantly comparing with huanglian alone,and there was an optimal ration among mateh-pair of huanglian-rougui.Conclusion:Chemical reaction in vitro and intestinal absorption were the key factors of match-pair of huanglian-rougui.更多还原