【作者】 王继明； 吴以岭； 李成仁；

【Author】 WANG Ji-ming~1,WU Yi-ling~1,LI Cheng-ren~2 (1.Yiling Hospital Affiliated Hebei Medical University,Shijiazhuang 050091,China; 2.Third Military Medical University,Chongqing 400038,China)

【机构】 河北医科大学附属以岭医院； 第三军医大学；

【摘要】 [目的]观察肌萎灵注射液(JWL)对人骨髓基质细胞增殖及向神经细胞分化的影响。[方法]应用密度梯度离心方法,体外分离培养人骨髓基质细胞,实验组施加不同浓度的肌萎灵注射液,对照组加等量培养基。应用液体闪烁法检测细胞增殖,免疫组织化学方法和流式细胞仪检测骨髓基质细胞分化后神经元特异中间丝蛋白(NF-200)和胶质纤维酸性蛋白(GFAP)的表达情况。[结果]肌萎灵注射液各浓度组人骨髓基质细胞生长较好,细胞增殖迅速,细胞计数显著提高,与对照组比较有显著性差异(P<0.01)。人骨髓基质细胞在加入含血清培养基的情况下,对照组自然分化成神经元和胶质细胞数目很少。肌萎灵注射液各剂量组NF-200和GFAP阳性细胞数均明显增多,与对照组有显著性差异(均P<0.01)。[结论]肌萎灵注射液明显促进人骨髓基质细胞的增殖和向神经细胞的分化。更多还原

【Abstract】 [Objective]To observe the effects of TCM Jiweiling Injection(JWL) on the proliferation and differentiation into nerve cells of bone marrow stromal cells.[Methods]The human marrow stromal cells(BMSCs) were isolated by density gradient centrifugation and primary cultured.Experimental groups were treated by JWL of different concentration diluting with fresh culture medium(0.1%、0.2%、0.5%、1%、2%、5%JWL) and control group was treated by fresh medium.The proliferation of the BMSCs was tested by the liquid scintillation counting after adulterating 3H-Thymidine(3H-TdR).Flow-cytometer technique and immunohistochemical staining were used to test the expression of neurofilament-200(NF-200) and glial fibrillary acidic protein(GFAP) after the differentiation of BMSCs.[Result]The human BMSCs grew better and proliferated faster in JWL groups and the CPM increased sharply by the the liquid scintillation counting after adulterating(3H-TdR),the difference comparing the control group was significant(P<0.01).The cell numbers that human BMSCs differentiated into neuron and gliocyte in control group after adding the serum culture medium were very slow which the ratios respectively were only (5.85±1.03)%and(8.90±1.44)%.The NF-200 and GFAP positive cells increased obviously in all JWL groups,the difference was significant comparing the control group(P<0.01).[Conclusion]JWL could promote the proliferation of the BMSCs and the differentiation into the neurons.更多还原