【作者】 刘雅婷； 李正跃； 郑元仙； 徐小刚； 李永忠；

【Author】 LIU Ya-ting~1,ZHENG Yuan-xian~1,LI Zheng-yue~2,XU Xiao-gang~1,LI Yong-zhong~3 (1.College of Agricultural and Biotechnology,Yunnan Agricultural University,Yunnan 650201,China; 2.College of plant protection,Yunnan Agricultural University,Yunnan 650201,China; 3.College of Tobacco Science,Yunnan Agricultural University,Yunnan 650201,China)

【机构】 云南农业大学农学与生物技术学院； 云南农业大学植保学院； 云南农业大学烟草学院；

【摘要】 Tospovirus属引起多种农作物和花卉的严重病害。本研究应用生物信息学软件DNAstar、DNAman对Tospovirus属所有成员的RNA-S中的外壳蛋白(NC)和非结构蛋白(NSs)的编码核酸和氨基酸序列进行了较为系统的分析:同源性树状图、进化树图以及一致性(Identity)的值,同源性的值以及进化树的值。同时应用网络软件NPSA和SMART进行了该蛋白质氨基酸组成和蛋白质拓扑结构的预测,发现ssRNA-S链中的NC的特异性非常高。为将来应用RT-PCR或IC-RT-PCR技术鉴定番茄斑萎病毒种的引物设计及致病机制研究提供依据。更多还原

【Abstract】 Tospovirus causes many kinds of serious diseases in crops and flowers.This research analyzed RNA-S sequence including the sequences of nucleocapsid protein(NC) and the non- structural protein(NSs) in all members of Tospovirus genus by bioinformation softwares(DNAstar and DNAman).Sequence compared charts(Homology tree, phylogenetic tree),and the value of Identity,homology and phylogenetic tree were obtained.Simultaneously,the protein amino acid composition and the protein topology structure were predicted by using network software NPSA and SMART.Specificity is extremely high and comparatively stable discovered in NC of ssRNA-S.It can be applied to design the primer to identify TSWV by RT-PCR and immunocapture-PCR.更多还原