【作者】 邹建军； 李绍臣； 王培忠；

【Author】 ZOU Jian-jun LI Shao-chen (Jinlin Provincial Academy of Forestry Science Changchun 130033) WANG Pei-zhong (The State Forestry Bureau manages in Changchun special commissioner Changchun 130022)

【机构】 吉林省林业科学研究院； 国家林业局驻长春专员办；

【摘要】 以简单玻璃化法为基本方法,研究了影响黄菠萝离体茎尖超低温保存的因子,建立了较为简单的黄菠萝离体茎尖超低温保存技术程序:取1.0cm的茎尖在含有80g/L蔗糖的1/2MS培养基上预培养2d,室温下LS装载液装载60min,然后用(PVS2)于0℃下处理30min,液氮保存至少1h后,在40℃水浴中化冻90s,用含80g/L蔗糖培养液洗涤,然后转入含80g/L蔗糖的MS培养基中,暗培养3d后转移到MS+1.0mg/L6-BA+0.1mg/LIBA培养基上,在光下进行再培养,再生植株生长和分化正常。更多还原

【Abstract】 The current paper studied factors that effect the cryopreservation of Phellodendron amurense Rupr. In vitro shoot tips.By using simple vitrification technique the rapid examination method of shoot tips survival was studied,and a sample procedure was established at last:Shoot tips about 1cm in length were precultured for 2d on 1/2MS medium supplemented with 80g/L sucrose.Dissected shoot apices were loaded with solution LS for 60min at room temperature.These excised shoot tips were sufficiently dehydrated in a highly concentrated plant vitrification solution (PVS2) for 30min at 0℃ and then plunged into liquid nitrogen and conserved for 1h.After rapid thawed in water at 40℃ for 90s.the tips were changed over to including 80g/L in the sucrose MS culture medium were raised darkly for three days, and then were plated on MS medium supplemented with 1.0mg/L 6-BA and 0.1mg/L IBA in the light were raised again, The regeneration plants were normal in Growth and differentiation .更多还原