【作者】 槐雅萍； 杨永轩； 贾新凤； 贾子善；

【Author】 HUAI Yaping Yang Yongxuan JIA Xinfeng JIA Zishan. Rehabilitation Center,Hebei Provincial People’s Hospital,Shijiazhuang,050051

【机构】 河北省人民医院康复中心；

【摘要】 目的:观察探索学习对局灶性脑梗死大鼠巢蛋白(nestin)表达的影响。方法:健康雄性SD大鼠70只,其中60只电凝法造成右侧大脑中动脉阻断(MCAO)模型后,随机分为探索学习组(n=30)居于探索笼,对照组(n=30)5只一组群居于标准笼,假手术组(n=10)仅开颅不电凝大脑中动脉,居于标准笼。探索学习组和对照组分别于术后1天、1周、2周、4周各组随机选取5只大鼠处死,假手术组分别于1周、4周时随机选取5只大鼠处死,即进行巢蛋白免疫组化染色,测定其在脑梗死灶周围皮质nestin的表达情况。结果:探索学习组nestin阳性神经元数在7天、14天明显多于手术对照组(P<0.05)。结论:探索学习能促进梗死灶周围皮层巢蛋白的表达。更多还原

【Abstract】 Objective:To study the effect of learning on nestin expression in rats after unilateral local cerebral infarction.Methods:70 male Sprague-Dawley rats were adopted.After making the model of MCAO by electric coagulation successfully,they were randomly divided into exploratory and learning group （n=30,living in exploratory cages）,control group（n=30,5 as a group living in large standard cages）.Other 10 rats as sham group living in standard cage.At the 1^st,7^th,14^th,28^th day after MCAO,5 rats were randomly killed separately in learning group and control group.5 rats in sham group were randomly killed separately at the 7^th,28^th day after operation.The expression of nestin in the peri-ischemic cortex were examined by using immunohistochemistry straining.Results:The number of nestin labeled cells in learning group was higher than those in control group at 7 and 14 day （P<0.05）.Conclusions:Learning could enhance nest in expression in rats after unilateral local cerebral infarction.更多还原