【作者】 槐雅萍； 贾新凤； 贾子善；

【Author】 HUAI Yaping JIA Xinfeng JIA Zishan

【机构】 河北省人民医院康复医学科；

【摘要】 目的:观察探索学习对局灶性脑梗死大鼠神经颗粒素(Ng)表达的影响。方法:健康雄性SD大鼠70只,其中60只电凝法造成右侧大脑中动脉阻断(MCAO)模型后,随机分为探索学习组(n=30)和对照组(n=30),假手术组(n=10)仅开颅不电凝大脑中动脉,居于标准笼。探索学习组和对照组分别于术后1天、1周、2周、4周各组随机选取5只大鼠处死,假手术组分别于1周、4周时随机选取5只大鼠处死,即进行Ng免疫组化染色,测定其在脑梗死灶周围皮质的表达情况。结果:探索学习组Ng阳性神经元数在7天、14天、28天明显多于手术对照组(P<0.01)。结论:探索学习能促进梗死灶周围皮层Ng表达。更多还原

【Abstract】 Objective:To study the effect of learning on Ng expression in rats after unilateral local cerebral infarction.Methods:70 male Sprague-Dawley rats were adopted.After making the model of MCAO by electric coagulation successfully,they were randomly divided into exploratory and learning group (n=30,living in exploratory cages),control group(n=30,5 as a group living in large standard cages).Other 10 rats didn’t been electric coagulated the middle cerebral artery,as sham group living in standard cage.At the 1st,7th,14th,28th day after MCAO,5 rats were randomly killed separately in learning group and control group.5 rats in sham group were randomly killed separately at the 7th,28th day after operation.The expression of Ng in the peri-ischemic cortex were examined by using immunohistochemistry straining.Results:The number of Ng labeled cells in learning group was higher than those in control group at 7,14 and 28 day (P<0.01).Conclusions:Learning could enhance Ng expression in rats after unilateral local cerebral infarction.更多还原