【作者】 蔡燕燕； 李茹； 赵燕芳； 陈欣； 朱沛华； 魏琴；

【Author】 CAI Yan-yan LI Ru ZHAO Yan-fang CHEN Xin ZHU Pei-hua WEI Qin School of Chemistry & Chemical Engineering,University of Jinan,Jinan 250022,China

【机构】 济南大学化学与化工学院；

【摘要】 研究了5,10,15,20-四-[对（叔丁氧羰苏氨酸）氨基苯基]卟啉（TAPP-Thr-Boc）作为荧光光谱探针用于含有L-色氨酸（L-tryptophan）残基的牛血清白蛋白（BSA）的定量测定。在实验中发现在AOT胶束存在下,蛋白质的加入可以猝灭TAPP-Thr-Boc的荧光强度,在pH为6.33的Briton-Robbison（B-R）缓冲溶液中,在最佳显色条件下测得BSA的线性范围为0.02～9.00μg·mL^-1,相关系数0.9829,检测限0.017μg·mL^-1。此法抗干扰能力强,有较好的选择性和灵敏度,用于实际样品奶粉的测定,结果满意。更多还原

【Abstract】 TAPP-Thr-Boc is selected as spectral probe for proteins determination by fluorescence method.The optimum condi- tions,such as acidity,dosage of buffer solution,media dosage and interfering ion are studied.Under the optimum conditions, the fluorescence of this system is in proportion to the concentration of BSA in the range 0.02-9.00μg·mL^-1,the correlation coefficient are 0.982 9,the detection limits are 0.017μg·mL^-1.The method mentioned in the paper has been successfully ap- plied to the determination of protein in milk powder.It can be seen that recovery and precision of this method are satisfactory.更多还原