碱性蛋白酶水解蓝圆鲹条件的优化研究

【作者】 陈申如；

【机构】 集美大学；

【Abstract】 The aim of this paper is to develop a process for hydrolysis Decapterus maruadsi with alkaline protease.The effects of the main parameters including hydrolysis temperature, pH value,hydrolysis time were investigated with Single Factor Experiment and dosage of enzyme,protein concentration were investigated with response surface methodology (RSM).The optimized conditions were:concentration of alkaline protease was 4320U/g protein,protein concentration was 1.57%,hydrolysis temperature was 55℃,hydrolysis pH value was 7.5,hydrolysis time was 6 hours;Under the conditions,amino acid nitrogen concentration attained 1.30mg/mL,hydrolysis degree attained 51.88%,recovery of the total nitrogen attained 97.07%and total volatile basic nitrogen reached 0.030mg/mL.RSD value of amino acid nitrogen concentration,hydrolysis degree and recovery of the total nitrogen was lower than 2%.更多还原