【作者】 常颖； 李先文；

【Author】 CHANG Ying,LI xian-wen (College of Science,Guangdong Ocean University,Zhanjiang 524088,China)

【机构】 广东海洋大学理学院；

【摘要】 本研究以黄斑海蜇（Rhopilema hispidum）下脚料为原料,采用酶水解法制备降血压肽。改进ACE（Angiotensin I-converting Enzyme,ACE）抑制率检测方法,并以ACE抑制率为指标,利用响应面法对酶解下脚料制备ACE抑制肽的工艺条件进行优化,在酶解条件（加酶量、液固比、酶解时间）和ACE抑制率之间建立了数学模型Y=65.404-2.486X₃+1.7X₂X₃-7.657X₁²-4.577X₂²-1.785X₃²。优化后的工艺参数为:加酶量0.92%、液固比1.95、酶解时间0.95h。根据回归方程的预测结果,其ACE抑制率达66.8%。更多还原

【Abstract】 Rhopilema hispidum offal was selected to prepare angiotens in converting enzyme inhibitors by enzymatic hydrolysis.And the ACE inhibitory activity analysis method was improved,the combined effects on ACE inhibitory activity production were studied using response surface methodology in this paper.The parameters were enzyme dosage,liquid-solid ratio and reaction time.The following regression equation was obtained:Y=65.404-2.486X₃+ 1.7X₂X₃-7.657X₁²-4.577X₂²-1.785X₃².The optimal combinations of media constituents for high ACE inhibitory activity were enzyme dosage 0.92%、iquid-solid ratio 1.95,reaction time 0.95h.Under the optimized conditions,the ACE inhibitory activity of the enzymatic hydrolysates was 66.8%更多还原