【作者】 刘海燕； 雷武； 朱晓鸣； 杨云霞； 韩冬； 薛敏； 解绶启；

【Author】 LIU Hai-yan1,2,3 LEI Wu1 ZHU Xiao-ming1 YANG Yun-xia1 HAN Dong1 XUE Min2 XIE Shou-qi1* (1 Institute of Hydrobiology,the Chinese Academy of Sciences,Wuhan,430072; 2 Feed Research Institute,the Chinese Academy of Agricultural Sciences,Beijing,100081; 3 Graduate school of the Chinese Academy of Sciences,Beijing 100039)

【机构】 中国科学院水生生物研究所淡水生态与生物技术国家重点实验室； 中国农业科学院饲料研究所； 河北师范大学生命科学学院；

【摘要】 为了探讨不同水平维生素C对长吻(?)Leiocassis longirostris Günther的影响,本实验在自然水温条件下,用维生素C(维生素C-2-聚磷酸酯)含量为38、364和630 mg/kg的三种饲料饲喂初体重为23.19±0.58g的长吻(?)8个月,结果表明,38mg/kg Vc饲料组饲喂的长吻(?)表现出了典型的Vc缺乏症状:体色发黑,脊椎侧弯,鳍边由开始的萎缩直到腐烂;贫血;特定生长率显著降低(p<0.05):血清溶菌酶活性显著下降(p<0.05);肝脏的SOD活性及MDA含量均显著升高(p<0.05);血清皮质醇没有显著差异(p>0.05);在各处理组的长吻(?)肿脏中均未检测到诱导型HSP70的存在。因此,皮质醇和HSP70不是Vc缺乏下营养胁迫的可靠指标,而血清溶菌酶、肝脏SOD活性和肝脏MDA含量,以及血液理化指标(血红细胞数及血红蛋白)更能代表Vc缺乏下的生理状态。更多还原

【Abstract】 Vitamin C is an essential micro-nutrient for most fishes and plays an important role in immunity and physiology.The deficiency of dietary vitamin C would result in the damage on fish.The study on the deficiency symptom and the sensitive indicator of vitamin C deficiency symptom could provide useful information for fish feed industry.This experiment was conducted to investigate the effects of dietary Vitamin C levels on growth performance, immune and physiological responses in Chinese longsnout catfish(Leiocassis longirostris Günther),which is an high value aquaculture fish in China.Three practical diets containing 38, 364 and 630 mg vitamin C/kg diet were fed to Chinese longsnout catfish(23.19±0.58g) for 8 months.Vitamin C were given as L-ascorbyl-2-polyphosphate.The results showed that the Chinese longsnout catfish in 38mg/kg vitamin C groups obtained typical vitamin C deficiency symptom:skin color turning darker,spinal deformities and scoliosis,erosion of fins,anaemia. SGR and the serum lysozyme activities decreased significantly(p<0.05),the hepatic SOD activities and MDA contents increased significantly(p<0.05).There was no significant difference in serum cortisol concentrations(p>0.05).The inductive HSP70 in liver was not detected by western-blotting in all groups.In conclusion,serum cortisol and hepatic HSP70 were not the sensitive indicators for vitamin C deficiency symptom and the serum lysozyme, hepatic SOD activity,hepatic MDA content and the haematological index(red blood cell number and hemoglobin content) were the more reliable makers to indicate the physiological status in Chinese longsnout catfish.更多还原