节点文献
三种群三角帆蚌杂交F1与其亲本的遗传差异
Molecular genetic variance between original parent and corresponding hybrids F1 of H.cumingii from three geographical population in china
【Author】 DONG Zhi-guo~(1,2) LI Jia-le~1 ZHENG Han-feng~(1,3) (1.The Key Laboratory of Aquatic Genetic Resources and Aquacultural Ecology Certificated by the Ministry of Agriculture,Shanghai Fisheries University,Shanghai 200090;2.Huihai Institute of Technolgy,Lianyungang,222005;3 East China Sea Fisheries Institute,Chinese Academy of Fisheries Science,Shanghai 200090,China)
【机构】 农业部水产种质资源与养殖生态重点开放实验室上海水产大学; 淮海工学院海洋学院; 中国水产科学研究院东海水产研究所;
【摘要】 采用40个随机引物对来自洞庭湖(DO)、鄱阳湖(PP)和太湖(TT)三个地理种群的三角帆蚌及其两两相互杂交所产生的6个正反交群体进行了随机扩增多态性DNA(RAPD)分析。40个随机引物中筛选出13个引物可产生稳定清晰的扩增带。每个引物的扩增带数为2~17条,扩增片段大小在200bp~3kbp之间,多态百分率在55%~100%之间。应用分子方差分析(AMOVA),发现群体间遗传变异为7.15%,群体内部的遗传变异为92.85%。群体间的遗传分化指数以洞庭湖与鄱阳湖种群正反交之间(DP和PD)(0.013)最小,其次为太湖♀×鄱阳湖♂(TP)和太湖亲本(TI)(0.020)以及太湖♀×鄱阳湖♂(TP)和太湖♀×洞庭湖♂(TD)(0.026),而以鄱阳湖♀×太湖♂(PT)和洞庭湖♀×太湖♂(DT)(0.165)最大,其次为太湖亲本(TT)和鄱阳湖♀×太湖♂(PT)(0.153),太湖♀×洞庭湖♂(TD)和鄱阳湖♀×太湖♂(PT)(0.150)以及洞庭湖亲本(DD)和鄱阳湖♀×太湖♂(PT)(0.137)最大。群体内的遗传多态度兀依次为:TP(0.335)>TD(0.3 17)>TT(0.308)>PD(0.300)>DP(0.298)>PP([0.297)>DT(0.291)>PT(0.290)>DD(0.269)。
【Abstract】 With the random amplified polymorphic DNA(RAPD)analysis method,forty ten-base arbitrary primers were used to study Hyriopsis cumingii genetic variance between original parent-the three geographical populations from Dongtinghu lake(DD),Poyanghu lake(PP)and Taihu lake(TT)in China and their corresponding 6 hybrids F1 populations.The amplified results of thirteen primers were stably and clearly endowed with abundant population polymorphy,and each primer could gain 2-17 fragments (200bp-3kb)in the 40 primers.Proportion of polymorphy was from 55% to 100%. AMOVA of 9 populations indicated that 7.15% genetic variance is from intra-population and 92.85% from inter-population.Fixation index(Fst),the three lowest were DP and PD(0.013),TP and TT(0.020)and TP and TD(0.026),the 4 highest as follows:PT and DT(0.165)>TT and PT(0.153)>TD and PT(0.150)>DD and PT(0.137). Inter-population gene diversity(π)is as follows:TP(0.335)>TD(0.317)>TT(0.308)>PD(0.300)>DP (0.298)>PP(0.297)>DT(0.291)>PT(0.291)>DD(0.269).
- 【会议录名称】 中国水产学会第五届青年学术年会摘要集
- 【会议名称】中国水产学会第五届青年学术年会
- 【会议时间】2004-11
- 【会议地点】中国上海
- 【分类号】S917.4
- 【主办单位】中国水产学会