【作者】 侯良芹； 徐婉莹； 戴岭； 施景； 耿结； 陆智敏； 邓银芬； 德伟；

【Author】 Hou Liang-qin Xu wan -ying Dai ling Shi Jing Geng Jie Lu Zhi-min Deng Yin-fen De Wei (Department of Biochemistry and Molecular Biology,nanjing Medical University,nanjing 210009)

【机构】 南京医科大学生物化学与分子生物学系；

【摘要】 目的:研究从核酸和蛋白两个方面对Mesothelin基因在胰腺发育不同阶段的表达和定位进行研究,并初步探讨该基因在胰腺发育过程中的生物学功能。方法:1.运用RT-PCR技术验证Mesothelin基因在大鼠胰腺发育不同阶段的mRNA表达水平。2.Western blot检测Mesothelin蛋白在大鼠胰腺发育不同阶段的表达丰度,3.用免疫荧光组织化学进行定位研究,4.最后运用实时荧光定量PCR技术对Mesothelin上游基因Wnt-5a在胰腺发育过程中的表达进行检测.结果:Mesothelin高表达的时期与胰岛结构形成和重塑的发育阶段相符,且定位于内分泌细胞,结论:1我们推测黏附分子Mesothelin可能调控胰腺胚胎发育后期胰岛细胞的迁移、聚集、黏附及胰岛结构的形成,并参与出生后胰岛结构重塑的过程,2其作用的发挥可能受上游因子Wnt-5a的调控,同时表达Mesothelin的胰腺间充质细胞可能是内分泌细胞的一个新的来源。更多还原

【Abstract】 Objective:This study was designed to investigate the expression and function of Mesothelin in development of rat pancreas.Methods:1.The mRNA expression levels of Mesothelin at different developmental stages were examined by RT-PCR.2.The abundance of Mesothelin protein in the rat pancreas during development were studied using western blot.3. Immunofluorescence is used to localization.4.At last,the expression of Wnt-Sa,upstream regulator of Mesothelin,was verified by real-time PCR.Results:the expression of Mesothelin concomitants with islets architecture formation and remodeling and that the Mesothelin is localized in islet cells.Conclusions 1.This suggested that Mesothelin might play a role in islet cells migration and adhesion during the process of islet architecture formation at the later gestation and in islet architecture remodeling and maturation after birth.2.The function of Mesothelin might be regulated by Wnt-Sa.Mesenchymal cells expressing Mesothelin simultaneously might be a potential source for endocrine cells.更多还原