【作者】 龙曼海； 蒋宪瑶； 张爱华； 邱燕；

【Author】 LONG Manhai~1,JIANG Xianyao~1,ZHANG Aihua~1,QIU Yan~2(1.Public Health college of Guiyang Medical college,Guiyang 550004,China;2.Nangtong market environmental momitoring centre station,Nangtong 226000,Jiangsu,China)

【机构】 贵阳医学院公共卫生学院； 江苏省南通市环境监测中心站；

【摘要】 目的通过特殊的细胞株研究多噻烷有无诱导芳香烃受体的作用。方法用先进的化学激活荧光素酶表达方法,离体测试多噻烷诱导完整细胞中芳香烃受体依赖的基因表达。结果多噻烷各浓度对人肝细胞瘤细胞有毒性,抑制细胞生长,对小鼠肝细胞瘤细胞基本无毒;各浓度在人和小鼠肝细胞瘤细胞中所致荧光素酶活性均未明显高于阴性对照组(DMSO)。结论多噻烷对人和小鼠肝细胞瘤细胞无诱导芳香烃受体的作用。更多还原

【Abstract】 Objectives To study if PCTA has the effect of inducing aromatic hydrocarbon receptor(AhR)-mediated.Method The in vitro CALUX(Chemical Active Luciferase eXpression) assay was used by employing the human and mouse hepatoma cell lines.Results The tested concentrations of PCTA were toxic to the human hepatoma TV101L cells,while they were not cytotoxic to the mouse hepatoma Hepa1.12cR cells.The luciferase activities of PCTA were not sifnificantly higher than control(DMSO) both in TV101L and Hepa1.12cR cell lines.Conclusion PCTA had no inducing effect of AhR-dependent fene expression both in human TV101L and mouse Hepa1.12cR cell lines.更多还原