【作者】 赵颖岚； 侯炜； 楮小刚； 陈文； 刘婧； 罗凡；

【Author】 ZHAO Ying-lan~1,HOU Wei~1, CHU Xiao-gang~2,et al.State Key Laboratory of Virology/Institute of Hrology,School of Medicine,Wuhan University(Wuhan 430071,China)

【机构】 病毒学国家重点实验室武汉大学医学病毒学研究所； 湖北省疾病预防控制中心武汉；

【摘要】 目的调查湖北汉族人群中人类白细胞抗原(HLA)-DRB1基因多态性与HIV易感或抵抗性的相关性。方法应用序列特异性引物聚合酶链反应(PCR-SSP)方法对湖北籍汉族64例无亲缘关系的HIV感染者进行HLA-DRB1基因型检测,分析健康对照者和HIV阳性感染者HLA-DRB1等位基因频率分布。结果在HIV阳性感染者中,HLA-DRB1 *0101-0103(Pc<0.01,RR=6.42)、*0401-0411(Pc<0.01,RR=21.93)、*1001(Pc<0.05, RR=10.43)的等位基因频率较对照组明显增高,提示可能与HIV易感性相关;而DRB1* 1501-1502(Pc<0.01,RR=0.06)、*1101-1105(Pc<0.01,RR=0.16)、*1301-1302(Pc<0.01, RR=0.10)、DRB4(Pc<0.01,RR=0.11)、DRB5(Pc<0.01,RR=0.10)的等位基因频率较对照组明显减少,提示可能与HIV的抗性有关。结论汉族人群中某些HLA-DRB1等位基因与HIV感染的易感性或抵抗性相关,这对阐明疾病的发生机制及HIV治疗和免疫预防、疫苗的研制有积极作用。更多还原

【Abstract】 Objective To investigate the relationship between genetic polymorphism of HLA-DRB1 and HIV-1 infection in Han population in Hubei province.Methods:A total of 64 HIV infected patients without blood relationship in Han population of Hubei province were selected and analyzed by using the polymerase chain reaction(PCR)with sequence-specific primers to test their HLA-DRB1 allele gene frequencies(GF),analyzing different distribution of HLA-DRB1 alleles in HIV~+ patients and HIV~- controls.Relative risk(RR)was applied to compare the likelihood of an event between two groups.Results There was a significantly higher frequency of DRB1*0101-0103,*0401-0411,*1001 allele among HIV infectious patients comparing controls,their RR were 6.42,21.93,10.43 respectively.They might be the susceptive alleles of HIV-1 infection.In contract,the gene frequency of DRB1*1501-1502,* 1101-1105,*1301-1302,DRB4,DRB5 in healthy controls were significantly higher than in the HIV-positive patients.And their RR were 0.06,0.16,0.10,0.11,0.10 respectively.They might be associated with resistance to HIV-1 infection.Conclusion Some HLA alleles in cohort study were associated with HIV-1 susceptibility or resistance.It will develop positive effect towards illuminating the mechanism of the disease,HIV treatment,immune prevention and vaccine development.更多还原