【作者】 王晓昆； 侯炜； 唐瑛； 杨占秋；

【Author】 WANG Xiao-kun,HOU Wei,TANG Ying,et al.Department of Medical Experiment,Wuhan General Hospital,PLA,Wuhan 430070,China

【机构】 广州军区武汉总医院医学实验科； 武汉大学医学院病毒研究所病毒学国家重点实验室；

【摘要】 目的:研究巨细胞病毒(CMV)感染、ET和IL-6三者在2型糖尿病(T2DM)发生与发展中的作用及其临床意义。方法:应用聚合酶链反应(PCR)检测110例T2DM患者及40例正常者外周血中的CMV-DNA,放射免疫法(RIA)测定血浆ET,双抗体夹心ELISA法测定血清IL-6的浓度。结果:T2DM组CMV感染率(59.09%)明显高于对照组(15.00%, P<0.01)。T2DM组血浆ET和血清IL-6浓度均明显高于对照组(P<0.01),T2DM组内CMV感染者的血浆ET和血清IL-6浓度均明显高于T2DM组中CMV未感染者和对照组(P<0.01)。结论:CMV感染、ET和IL-6可能在T2DM的发生、发展中起重要作用。更多还原

【Abstract】 Objective:To investigate the relationship between cytomegalovirus(CMV) infection,plasma endothelin(ET),serum interleukin-6(IL-6)in patients with Type 2 Diabetic Mellitus(T2DM).Methods:Blood samples were collected from 110 T2DM patients and 40 normal controls(NC).CMV-DNA was detected by polymerase chain reaction(PCR).Plasma ET and serum IL-6 were examined by radio-immunological assay(RIA)and double-antibodies ELISA respectively.Results:CMV infection rate in the T2DM group(59.09%)was significantly higher than NC group(15.00%,P<0.01).Concentrations of plasma ET and serum IL-6 in T2DM were both higher than those of NC group(all P<0.01).And concentrations of plasma ET and serum IL-6 in T2DM with CMV infection were significant higher than T2DM without CMV infection and NC group(P<0.01).Conclusion:CMV infection,ET and IL-6 might be closely linked with T2DM and implicated in the pathogeneses of T2DM.更多还原