【作者】 缪旻珉； 张宗东； 程皓； 王奎山；

【机构】 扬州大学园艺与植物保护学院；

【摘要】 根据其他物种中α-半乳糖苷酶序列的保守区段设计引物,综合运用反转录聚合酶链式反应(RT- PCR)和快速扩增cDNA末端(RACE)等技术,发现并克隆了4种黄瓜α-半乳糖苷酶的全长cDNA序列,分别命名为:黄瓜酸性α-半乳糖苷酶Ⅰ(DQ320569)、黄瓜酸性α-半乳糖苷酶Ⅱ(DQ361277)、黄瓜碱性α-半乳糖苷酶Ⅰ(DQ157703)和黄瓜碱性α-半乳糖苷酶Ⅱ(DQ395330)。这项工作使黄瓜成为迄今为止发现并克隆α-半乳糖苷酶基因种类最多的植物,为进一步研究该酶在棉子糖家族寡糖(RFOs)运输型植物中的生理功能奠定了良好的基础。更多还原

【Abstract】 4 full length cDNA sequences ofα-galactosidases were obtained by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and Rapid Amplification of cDNA Ends (RACE) in cucumber,according to homological sequences ofα-galactosidase genes in other plants.They are named:cucumber acidα-galactosidasesⅠ(DQ320569),cucumber acidα-galactosidasesⅡ(DQ361277),cucumber alkalineα-galactosidasesⅠ(DQ157703) and cucumber alkalineα-galactosidasesⅡ(DQ395330).To our knowledge,this is the first report of cloning full length cDNAs of 2 acid and 2 alkalineα-galactosidases in the same plant.Thus,We can design probes,primers or RNA interference based on these sequences to do further research work in this area.更多还原