【作者】 许晔； 施国新； 徐勤松； 王学； 丁秉中；

【Author】 Xu Ye Shi Guoxin Xu Qinsong Wang Xue Ding Bingzhong(College of Life Sciences,Nanjing Normal University,Nanjing 210097,P.R.China)

【机构】 南京师范大学生命科学学院；

【摘要】 实验以高等水生植物茶菱（Trapella Sinensis Olive）为材料,研究了外施不同浓度的脯氦酸（Pro） 对 Cd²⁺毒害的缓解效应。结果表明,茶菱体内 Pro 含量在单一 Cd²⁺毒害下明显增加,外施 Pro 后,显著降低；叶绿素和可溶性蛋白含量在单一 Cd²⁺毒害下大幅下降,外施 Pro 后,失绿症状有所减轻：保护酶（SOD、 CAT、POD）系统在单一 Cd²⁺毒害下比例失调,平衡被打破,活性下降,外施 Pro 后,SOD,POD 活性较对照升高,CAT 持平,其比例维持在正常范围内；O₂产生速率在单一 Cd²⁺毒害下大大加快,外施 Pro 后, 其产生速率恢复正常水平；丙二醛（MDA）含量在单一 Cd²⁺毒害下积累加剧,外施 Pro 后,积累程度有所降低。因此,外源 Pro 可以通过减轻叶绿素和可溶性蛋白的降解,促进蛋白合成,减缓 MDA 积累,降低 O₂产生速率,增强保护酶活性来增强植物对重金属胁迫的抗性,增强植物对逆境的适应能力。本实验中 Pro 作用的最佳浓度范围为40～60mg·L^-1。更多还原

【Abstract】 The mitigation of toxic effect of Cd²⁺ on Trapella Sinensis Olive by exogenous Pro was studied.The results show that when treated with single 3mg·L^-1Cd²⁺for 5d.the Pro content increased,chlorophyll and soluble protein content droped,the balance of antioxidase（SOD,POD,CAT）was broken and the activity of their decreased, MDA content,O₂ generation rate increased in Trapella Sinensis Olive.When different conditions of Pro was added into the solution,Pro content droped,the degradation of chlorophyll and soluble protein was alleviated,the activity of SOD and POD raised.the activity of CAT returned to normal level,the balance of antioxidase was rebuilded,the activity of antioxidase was enhanced,O₂ generation rate was droped,and accumulation of MDA was reduced in comparison with single Cd²⁺ treatment.As a result of that exogenous Pro can enhance the resistance of plants to heavy metal stress.The optimumdose of Pro to Cd²⁺in Trapella Sinensis Olive was ranged from 40～60 mg·L^-1.更多还原