【作者】 汪立平； 张庆华； 赵勇； 陈有容； 齐凤兰； 张闻；

【Author】 Wang Liping Zhang Qinhua Zhao Yong Chen Yourong Zhang Wen (Shanghai Fisheries University,Shanghai 200090)

【机构】 上海水产大学；

【摘要】 从五种变质豆浆中分离得到三株菌株 S1、S2和 S3。三株腐败菌均能在121℃,30min 杀菌条件下和添加300mg/kgNisin 杀菌条件下存活。对分离菌株进行了菌落形态、生理生化特征和16S rDNA 序列分析,鉴定结果表明,三株菌株均为细菌,革兰氏染色阴性,S1、S2和 S3分别为地衣芽孢杆菌(Bacillus licheniformis)、短小芽孢杆菌(Bacillus pumilus)、短芽孢杆菌(Brevibacillus borstelensis)。三株菌株 GenBank 登录号为 EF439666-EF439668。更多还原

【Abstract】 In this paper,three spoilage organisms were separated from five transmutative soy milks,and all the three spoilage bacteria could survive condition of both 121℃,30min and 300mg/kg Nisin.Morpha character,physiological and biochemical characteristics,and a phylogenetic analysis based on 16S rDNA gene sequences reveal that these three strains are Bacillus licheniformis,Bacillus pumilus and Brevibacillus borstelensis respectively.GenBank accessions for these three strains are EF439666-EF439668。更多还原