【作者】 张海科； 范志金； 马琳； 贾俊超； 黎锐；

【Author】 Hai-Ke Zhang, Zhi-Jin Fan*, Lin Ma, Jun-Chao Jia, Rui Li State Key Laboratory of Elemento Organic Chemistry, Nankai University, Tianjin 300071

【机构】 南开大学元素有机化学国家重点实验室；

【摘要】 <正>绿色荧光蛋白(GFP)是海洋生物水母(Aequorea victoria)体内的生物发光蛋白,分子量为26888D,由238个氨基酸组成,该蛋白65～67位Ser-Tyr-Gly三种氨基酸环化加氧形成特殊的生色团结构。更多还原

【Abstract】 Green fluorescent protein (GFP) is a bioluminescent protein coming from the jelleyfish Aequorea victoria. The Aequrea GFP is composed of 238 amino acid residue with a calculated molecular weight of 26888D. The highly fluorescent chromophore is composed of a modification of the Ser-Tyr-Gly at positions 65～67 within the polypeptide by oxidative cyclyzation. These three residues are cyclized and oxidized to form the phydroxybenzylideneimidazolidinone chromophore. The expression of wild-type GFP in stably transformed plants has typically yielded very faint or no green fluorescence. The engineered gfp gene was resynthesized to adapt its codon usage for expression in animals and plants by increasing G or C content to mutate the cryptic intron for escaping the aberrant mRNA processing. The modified GFP versions were used as a reporter gene in place of GUS gene, as a marker gene and as a subcellular targeting tool in animals and plants. In addition, GFP was effective in the study on ecological monitoring and interactions between microbes and plants, it is also a very important tool for novel medicine screening and target identification.更多还原