【作者】 邵峰； 刘荣华； 马志林； 陈星； 杨明； 廖正根；

【Author】 SHAO Feng LIU Rong-hua~* Ma Zhi-lin CHEN Xing YANG Ming LIAO Zheng-gen (Key Laboratory of Modem Preparation of TCM(Jiangxi University of Traditional Chinese Medicine),Ministry of Education,Nanchang 330004,China)

【机构】 江西中医学院现代中药制剂教育部重点实验室；

【摘要】 目的:优化山楂叶总黄酮提取纯化工艺。方法:通过比色法进行总黄酮含量测定,采用正交试验设计,对乙醇提取工艺及 HP-20、HPD-200、HPD-500型大孔吸附树脂纯化工艺进行筛选和优化。结果:最佳提取工艺:采用70%乙醇提取三次,70℃的水浴加热,每次用10倍量的乙醇提取1h。在此提取工艺条件下,总黄酮得率最高;最佳纯化工艺:用 HPD-500 型大孔吸附树脂进行吸附,70%乙醇洗脱。在此纯化工艺条件下,山楂叶总黄酮纯度达到85%以上,且转移率达到72%以上。结论:优化确定了山楂叶总黄酮提取纯化工艺。更多还原

【Abstract】 OBJECTIVE:To determine the optimal parameters for the extraction and purification process of total flavonoid from Hawthorn leaves.METHODS:Total flavonoid of Hawthorn leaves was determined by colorimetry.It was investigated by the orthogonal experiment design of form L₉（3⁴） with the extraction process by alcohol and the purification process by screening three types of macroporous resin,which were HP-20,HPD-200 and HPD-500.RESULTS:The optimal condition of extraction was with 70% alcohol as much as ten times of medical material amount and was bathed for 1 h and three times in 70℃ water.Under these conditions,the extraction ratio of total flavonoid from Hawthorn leaves was highest.The optimal condition of purification was as follows:HPD-500 macroporous resin as absorbents,and 70% alcohol as eluant.Under these conditions,the purification ratio of total flavonoid from Hawthorn leaves was more than 85%.The transition ratio was more than 72%.CONCLUSION:The optimal parameters for the extraction and purification process of total flavnoids from Hawthorn leaves were determined.更多还原