【作者】 吴黎； 熊承良； 苏萍；

【Author】 WuLi XiongChenLiang SuPing~#(Family Planning Research Institute,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China)

【机构】 华中科技大学同济医学院计划生育研究所；

【摘要】 目的:探讨哇巴因在妊娠高血压疾病发生发展中的作用。方法:以30例正常晚期妊娠妇女为对照组,30例妊娠高血压疾病患者为病例组:病例组分为妊娠期高血压组、轻度子痫前期组、重度子痫前期组。采用 SP 法、硝酸还原酶法和 ELISA 法分别检测哇巴因在胎盘组织的表达以及孕妇外周血和新生儿脐血中 NO 和 ET-1的含量:结果:①病例组各组哇巴因在胎盘组织的表达不仅显著高于对照组,并且随着疾病的加重,表达强度逐渐增强 (P<0.01)。②哇巴因的表达与 ET-1正相关(r=-0.5567,P<0.01),与 NO 负相关(r=0.6895, P<0.01);ET-1与 NO 呈明显的负相关(r=-0.7796,P<0.01)。③病例组孕妇外周血/新生儿脐血的 ET-1水平显著高于对照组(P<0.01);而 NO 水平明显低于对照组(P<0.01)。病例组和对照组的脐血 ET-1水平均显著高于孕妇外周血(P<0.01);NO 水平明显低于孕妇外周血(P<0.01)。结论:哇巴因在妊娠高血压疾病患者胎盘组织中随着血压升高而表达增强, 且哇巴因的表达与 ET1/NO 的失衡有显著的相关性,提示:哇巴因可能参与妊娠高血压疾病的发生发展,哇巴因对妊娠高血压疾病的病理调控与降低 NO 和升高 ET-1有关。更多还原

【Abstract】 The expression of endogenous ouabain in placenta and the serum ET-1 and NO concentrations were examined in 30 patients with hypertensive disorder complicating pregnancy and 30 healthy pregnant women for the purpose of investigate the effective of endogenous ouabain with hypertensive disorder complicating pregnancy.Compared with the healthy pregnant group, the expression of endogenous ouabain were dramatically increased in the hypertensive disorder complicating pregnancy groups(P<0.01).There was a significant positive correlation between the expression of endogenous ouabain with ET-1(r=-0.5567,P<0.01),while there was a significant negative correlation between the expression of endogenous ouabain with NO (r=0.6895,P<0.01)and the same between ET-1 with NO(r=-0.7796,P<0.01).The concentrations of ET-1 of maternal serum and cord serum in hypertensive disorder complicating pregnancy groups were significantly high in comparison with healthy pregnant group(P<0.01).But the opposite between the hypertensive disorder complicating pregnancy groups with healthy pregnant group on the Maternal and cord serum NO concentrations(P<0.01).ET-1 concentrations in cord serum were significantly high in comparison with maternal serum(P<0.01).Meanwhile,the NO level in cord serum were significantly lower in maternal serum in all the groups(P<0.01).These findings demonstrate that the expression of endogenous ouabain,as well as the advanced blood hypertension,was reinforced gradually.And there was a significant correlation between the expression of EO with the disequilibrium of ET-1 and NO.Which suggested that endogenous ouabain may participated the pathogenesis of hypertensive disorder complicating pregnancy.更多还原