【作者】 王斌； 曹三杰； 文心田； 黄小波；

【Author】 Wang Bin CAO San-jie WEN Xin-tian HUANG Xiao-bo (Laboratory of Animal Infectious Disease and Microarray;Key Laboratory of Animal Disease and Human Health of Sichuan Province,College of Veterinary Medicine,Sichuan Agricultural University,Yaan 625014,China)

【机构】 四川农业大学动物医学院动物传染病与基因芯片实验室动物疫病与人类健康四川省重点实验室；

【摘要】 采用超速离心和阴离子交换层析等方法提取制备了 App 血清1至10型等10个血清型的荚膜多糖和脂多糖抗原,建立了检测 App 型特异性抗体的 ELISA 方法,试验结果为:以 App 血清1型和4型的荚膜多糖及2型和10型的脂多糖为诊断抗原建立的 ELISA 方法,只与相应血清型的 App 标准阳性血清呈阳性反应, 与其它 App 血清型标准阳性血清无交叉反应;其它6种 App 血清型的两种抗原与异型 App 阳性血清之间具有不同程度的交叉反应。结果表明,两种诊断抗原均可用于胸膜肺炎放线杆菌型特异性抗体的检测,尤以 App 血清1型和4型的荚膜多糖及2型和10型的脂多糖抗原最佳。更多还原

【Abstract】 CPS(capsular polysaccharide)and LPS(lipopolysaccharide)were extracted from App(Actinobacillus Pleuropneumoniae)serotype 1 to serotype 10 by ultra-centrifugalization and anion exchange column.Indirect ELISA was developed by these two antigens.The result is that CPS from App serotype 1 and 4 ,LPS from App serotype 2 and 10 only have reaction with antisera to App corresponding serotype and have no cross reaction with antisera to other App serotype.The CPS and LPS from other 6 serotype have the different level corss-reaction with antisera to other App serotype.It was indicated that these two kinds of antigens from App both could be used to detect serotype specific antibody of App,especially the CPS from serotype 1 and serotype 4,LPS from serotype 2 and 10.更多还原