【作者】 张义平； 陈学峰； 熊建华； 朱英国；

【Author】 ZHANG Yi-Ping CHEN Xue-Feng XDNG Jian-Hua ZHU Ying-Guo~* (Key Laboratory of MOE for Plant develapmental Biology, Wuhan University, Wuhan 430072,China)

【机构】 武汉大学植物发育生物学教育部重点实验室；

【摘要】 运用 DDRT-PCR 对红莲型杂交稻组合红莲优6号及其亲本、保持系(粤泰 A、粤泰 B、9311)的一叶期、三叶期叶片基因表达状况进行分析。结果表明:杂种与亲本之间在同一发育时期基因表达既有质量上又有数量上的差异,但差异表达基因所占比例较小;而对于同一材料,一叶期、三叶期的叶片 cDNA 扩增带型相似没有发现基因质上的差异表达,说明一叶期与三叶期幼苗叶片基因表达差异很小。实验也证明 DDRT-PCR 技术结合银染方法是一种简单快速分析差异表达基因的有效方法。更多还原

【Abstract】 The gone expression in leaves for hybrid rice, Honglianyou-6, and its parental lines (YTA, YTB, 9311) at different development stages was analyzed by using DDRT-PCR. Our re- sults revealed that both quality and quantity difference in gene. expression were detected among hybrid and parental lines at same developmental stage. Although gone expression in hybrid is ob- viously altered, the differential expressed gene still occupy a very small proportion of total ex- pressed gene; In addition, no qualitative difference in gene expression was observed between one- leaf stage and tri-leaf stage in the same material This indicate the great sin ilarity of gene exp res- sion in the two stages Also,our experiment further provide evidence that DDRT-PCR technique with silver staining. is a rap id, and convenient method for detecting differentially expressed genes更多还原